Activation of synovial fibroblasts from patients at revision of their metal-on-metal total hip arthroplasty
| Autor: | Julia C. Shelton, Yuanhao Wu, Jing Xu, Jian Chen, Xiaoli Zhang, Junyao Yang, Agata Nyga, Alister Hart |
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| Rok vydání: | 2020 |
| Předmět: |
Chromium
Male Pathology medicine.medical_specialty Health Toxicology and Mutagenesis Arthroplasty Replacement Hip lcsh:Industrial hygiene. Industrial welfare Periprosthetic Inflammation Matrix (biology) Synovial fibroblast Toxicology Cobalt chromium debris 03 medical and health sciences lcsh:RA1190-1270 Fibrosis medicine Metallosis Humans lcsh:Toxicology. Poisons Aged 0303 health sciences Chemistry Macrophages Research Synovial Membrane 030311 toxicology Soft tissue General Medicine Cobalt Fibroblasts Middle Aged medicine.disease Cell culture Metals Monocyte differentiation Metal-on-Metal Joint Prostheses Female medicine.symptom lcsh:HD7260-7780.8 |
| Zdroj: | Particle and Fibre Toxicology Particle and Fibre Toxicology, Vol 17, Iss 1, Pp 1-13 (2020) |
| ISSN: | 1743-8977 |
| Popis: | Background The toxicity of released metallic particles generated in metal-on-metal (MoM) total hip arthroplasty (THA) using cobalt chromium (CoCr) has raised concerns regarding their safety amongst both surgeons and the public. Soft tissue changes such as pseudotumours and metallosis have been widely observed following the use of these implants, which release metallic by-products due to both wear and corrosion. Although activated fibroblasts, the dominant cell type in soft tissues, have been linked to many diseases, the role of synovial fibroblasts in the adverse reactions caused by CoCr implants remains unknown. To investigate the influence of implants manufactured from CoCr, the periprosthetic synovial tissues and synovial fibroblasts from patients with failed MoM THA, undergoing a revision operation, were analysed and compared with samples from patients undergoing a primary hip replacement, in order to elucidate histological and cellular changes. Results Periprosthetic tissue from patients with MoM implants was characterized by marked fibrotic changes, notably an increase in collagen content from less than 20% to 45–55%, an increase in α-smooth muscle actin positive cells from 4 to 9% as well as immune cells infiltration. Primary cell culture results demonstrated that MoM synovial fibroblasts have a decreased apoptosis rate from 14 to 6% compared to control synovial fibroblasts. In addition, synovial fibroblasts from MoM patients retained higher contractility and increased responsiveness to chemotaxis in matrix contraction. Their mechanical properties at a single cell level increased as observed by a 60% increase in contraction force and higher cell stiffness (3.3 kPa in MoM vs 2.18 kPa in control), as measured by traction force microscopy and atomic force microscopy. Further, fibroblasts from MoM patients promoted immune cell invasion by secreting monocyte chemoattractant protein 1 (MCP-1, CCL2) and induced monocyte differentiation, which could also be associated with excess accumulation of synovial macrophages. Conclusion Synovial fibroblasts exposed in vivo to MoM THA implants that release CoCr wear debris displayed dramatic phenotypic alteration and functional changes. These findings unravelled an unexpected effect of the CoCr alloy and demonstrated an important role of synovial fibroblasts in the undesired tissue reactions caused by MoM THAs. |
| Databáze: | OpenAIRE |
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