Anti-inflammatory Effects of Oct4/Sox2-overexpressing Human Adipose Tissue-derived Mesenchymal Stem Cells
| Autor: | Hwa-Young Youn, Min-Ok Ryu, Sei-Myoung Han, Sang Chul Park, Qiang Li, Woo-Jin Song |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Adipose tissue macrophages Green Fluorescent Proteins Anti-Inflammatory Agents Adipose tissue environment and public health General Biochemistry Genetics and Molecular Biology Mice 03 medical and health sciences SOX2 parasitic diseases Animals Humans Medicine Cells Cultured Stem cell transplantation for articular cartilage repair Inflammation Pharmacology business.industry SOXB1 Transcription Factors fungi Mesenchymal stem cell Mesenchymal Stem Cells Amniotic stem cells Survival Rate RAW 264.7 Cells 030104 developmental biology Adipose Tissue embryonic structures Cancer research Cytokines biological phenomena cell phenomena and immunity Stem cell business Octamer Transcription Factor-3 Research Article Adult stem cell |
| Zdroj: | In Vivo. 31:349-356 |
| ISSN: | 1791-7549 |
| Popis: | Background/aim The transcription factors Oct4 and Sox2 enhance the proliferation and pluripotency of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs); however, the anti-inflammatory effects of Oct4- and Sox2-overexpressing hAT-MSCs (Oct4/Sox2-hAT-MSCs) are unclear. Here, we evaluated the anti-inflammatory effects of Oct4/Sox2-hAT-MSCs in vitro and in vivo. Materials and methods Supernatants from green-fluorescent protein (GFP)- and Oct4/Sox2-hAT-MSCs were used to treat lipopolysaccharide (LPS)-stimulated RAW264.7 cells and inflammatory cytokine expression was determined. In LPS-induced mice, GFP- and Oct4/Sox2-hAT-MSCs were injected intraperitoneally and survival rates, as well as sickness scores of mice, were monitored. Results Decreased expression of pro-inflammatory cytokines was observed in Oct4/Sox2-hAT-MSC supernatant-exposed RAW264.7 cells compared to that in GFP-hAT-MSC supernatant-exposed RAW264.7 cells. The sickness score was reduced to 34.9% and the survival rate was increased by 11.1% in Oct4/Sox2-hAT-MSC-injected mice compared to that in GFP-hAT-MSC-injected mice. Conclusion Our findings provide important insights into the development of therapies utilizing Oct4/Sox2-hAT-MSCs in inflammatory diseases. |
| Databáze: | OpenAIRE |
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