Nascent RNA structure modulates the transcriptional dynamics of RNA polymerases
| Autor: | Lacramioara Bintu, Carlos Bustamante, Bradley M. Zamft, Toyotaka Ishibashi |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Myxococcus xanthus
RNA Folding Saccharomyces cerevisiae Proteins Transcription Genetic RNA-induced transcriptional silencing Oligonucleotides RNA-dependent RNA polymerase Saccharomyces cerevisiae Models Biological Polymerase Chain Reaction Mitochondrial Proteins chemistry.chemical_compound RNA polymerase RNA polymerase I Computer Simulation Polymerase Base Composition Multidisciplinary biology RNA DNA-Directed RNA Polymerases Biological Sciences Non-coding RNA Molecular biology Cell biology chemistry biology.protein RNA Polymerase II Small nuclear RNA |
| Zdroj: | Proceedings of the National Academy of Sciences. 109:8948-8953 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1205063109 |
| Popis: | RNA polymerase pausing represents an important mechanism of transcriptional regulation. In this study, we use a single-molecule transcription assay to investigate the effect of template base-pair composition on pausing by RNA polymerase II and the evolutionarily distinct mitochondrial polymerase Rpo41. For both enzymes, pauses are shorter and less frequent on GC-rich templates. Significantly, incubation with RNase abolishes the template dependence of pausing. A kinetic model, wherein the secondary structure of the nascent RNA poses an energetic barrier to pausing by impeding backtracking along the template, quantitatively predicts the pause densities and durations observed. The energy barriers extracted from the data correlate well with RNA folding energies obtained from cotranscriptional folding simulations. These results reveal that RNA secondary structures provide a cis -acting mechanism by which sequence modulates transcriptional elongation. |
| Databáze: | OpenAIRE |
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