Self-assembled fluorescent Ce(Ⅲ) coordination polymer as ratiometric probe for HIV antigen detection
| Autor: | Yalan Liu, Ningxing Li, Songbai Tian, Xinghu Ji, Mingyuan Du, Qinxue Hu, Guobin Mao, Yucheng Liu, Zhike He, Xinxin Wang |
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| Rok vydání: | 2019 |
| Předmět: |
HIV Antigens
Polymers Coordination polymer viruses Human immunodeficiency virus (HIV) HIV Infections 02 engineering and technology medicine.disease_cause 01 natural sciences Biochemistry Analytical Chemistry chemistry.chemical_compound Antigen Coordination Complexes medicine Humans Environmental Chemistry Fluorescein Hydrogen peroxide Spectroscopy Fluorescent Dyes Detection limit 010401 analytical chemistry Cerium 021001 nanoscience & nanotechnology Fluorescence 0104 chemical sciences chemistry Capsid Biophysics 0210 nano-technology |
| Zdroj: | Analytica Chimica Acta. 1084:116-122 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/j.aca.2019.08.010 |
| Popis: | The viral capsid protein p24 of human immunodeficiency virus is expressed at different level during viral invasion. Detection of p24 is of great importance in acquired immunodeficiency syndrome monitoring and therapy. A ratiometric probe that is easily-synthesized was constructed based on self-assembled fluorescent Ce(Ⅲ) and fluorescein. Fluorescein was used as reference. Hydrogen peroxide quenches the fluorescence of the Ce(III) easily but does not quench the fluorescence of fluorescein. The mechanism of reaction was discussed. Benefiting from the sensitive response to hydrogen peroxide, this probe was applied for p24 detection in enzyme linked immunoassay. The fluorescence ratio was in a good linear relationship with the concentration of p24, and the detection limit was 1.1 pg mL−1. This proposed method has shown potential in virus detection with easy operation. |
| Databáze: | OpenAIRE |
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