Prostaglandin E(2)-loaded microspheres as strategy to inhibit phagocytosis and modulate inflammatory mediators release
| Autor: | Peter J. Jose, Roberto Nicolete, Karla De Melo Lima, Maria-Jesus Sanz, Lúcia Helena Faccioli, José Maciel Rodrigues Júnior |
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| Rok vydání: | 2007 |
| Předmět: |
Time Factors
medicine.medical_treatment Phagocytosis Chemistry Pharmaceutical Drug Compounding Pharmaceutical Science Inflammation Pharmacology Biology Nitric Oxide Dinoprostone chemistry.chemical_compound Mice Immune system In vivo Sepsis medicine Animals Humans Immunologic Factors Prostaglandin E2 Particle Size Cells Cultured Chemokine CCL2 Tumor Necrosis Factor-alpha Endothelial Cells Water General Medicine Microspheres Disease Models Animal chemistry Biochemistry Solubility Delayed-Action Preparations Macrophages Peritoneal Liberation lipids (amino acids peptides and proteins) Arachidonic acid Emulsions medicine.symptom Inflammation Mediators Oils Biotechnology Prostaglandin E medicine.drug |
| Zdroj: | European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V. 70(3) |
| ISSN: | 0939-6411 |
| Popis: | PGE(2), an arachidonic acid metabolite produced by various type of cells regulates a broad range of physiological activities in the endocrine, cardiovascular, gastrointestinal, and immune systems, and is involved in maintaining the local homeostasis. In the immune system, PGE(2) is mainly produced by APCs and it can suppress the Th1-mediated immune responses. The aim of this study was to develop PGE(2)-loaded biodegradable MS that prolong and sustain the in vivo release of this mediator. An o/w emulsion solvent extraction-evaporation method was chosen to prepare the MS. We determined their diameters, evaluated the in vitro release of PGE(2), using enzyme immunoassay and MS uptake by peritoneal macrophages. To assess the preservation of biological activities of this mediator, we determined the effect of PGE(2) released from MS on LPS-induced TNF-alpha release by murine peritoneal macrophages. We also analyzed the effect of encapsulated PGE(2) on inflammatory mediators release from HUVECs. Finally, we studied the effect of PGE(2) released from biodegradable MS in sepsis animal model. The use of this formulation can provide an alternative strategy for treating infections, by modulating or inhibiting inflammatory responses, especially when they constitute an exacerbated profile. |
| Databáze: | OpenAIRE |
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