Study on the distribution sites and the molecular mechanism of analgesia after intracerebroventricular injection of rat/mouse hemokinin-1 in mice
| Autor: | Yuanting Jin, Caiyun Fu, Xia Ruilong, Zhao Fukun, Shi-Fu Zhang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Pro-Opiomelanocortin Physiology medicine.drug_class Narcotic Antagonists Endogeny Peptide Biochemistry Mice Cellular and Molecular Neuroscience Endocrinology Neurokinin-1 Receptor Antagonists Proopiomelanocortin Opioid receptor Tachykinins Internal medicine medicine Animals RNA Messenger Receptor chemistry.chemical_classification Mice Inbred ICR Messenger RNA biology Antagonist Receptors Neurokinin-1 Infusions Intraventricular Opioid Peptides chemistry Receptors Opioid biology.protein Female NK1 receptor antagonist Analgesia |
| Zdroj: | Peptides. 43:113-120 |
| ISSN: | 0196-9781 |
| DOI: | 10.1016/j.peptides.2013.02.020 |
| Popis: | Hemokinin-1 is a peptide encoded by Pptc, which belongs to the family of mammalian tachykinins. Our previous results showed that rat/mouse hemokinin-1 (r/m HK-1) produced striking analgesia after intracerebroventricular (i.c.v.) injection in mice, and the analgesia could be blocked by the NK1 receptor antagonist and the opioid receptor antagonist, respectively. However, the precise distribution sites and the molecular mechanism involved in the analgesic effect after i.c.v. administration of r/m HK-1 are needed to be further investigated deeply. Using the fluorescence labeling method, our present results directly showed that r/m HK-1 peptides were mainly distributed at the ventricular walls and several juxta-ventricular structures for the first time. Our results showed that the mRNA expressions of NK1 receptor, PPT-A, PPT-C, KOR, PDYN, DOR and PENK were not changed markedly, as well as the protein expression of NK1 receptor was hardly changed. However, both the transcripts and proteins of MOR and POMC were up-regulated significantly, indicating that the analgesic effect induced by i.c.v. administration of r/m HK-1 is related to the activation of NK1 receptor first, then it is related to the release of endogenous proopiomelanocortin, as well as the increased expression level of μ opioid receptor. These results should facilitate further the analysis of the analgesia of r/m HK-1 in the central nerval system in acute pain and may open novel pharmacological interventions. |
| Databáze: | OpenAIRE |
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