The nuclear genes encoding the internal (KlNDI1) and external (KlNDE1) alternative NAD(P)H:ubiquinone oxidoreductases of mitochondria from Kluyveromyces lactis
| Autor: | M.E. Cerdán, S. Díaz Prado, M.I. González Siso, Nuria Tarrío |
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| Rok vydání: | 2005 |
| Předmět: |
Glucose-6-phosphate isomerase
Nuclear gene Transcription Genetic Molecular Sequence Data Saccharomyces cerevisiae Mutant Biophysics Biology Pentose phosphate pathway Biochemistry Fungal Proteins Mitochondrial Proteins Pentose Phosphate Pathway Kluyveromyces Gene Expression Regulation Fungal Alternative dehydrogenase Amino Acid Sequence Cloning Molecular Gene Kluyveromyces lactis Electron Transport Complex I NAD(P)H:ubiquinone oxidoreductase Cell Biology biology.organism_classification Molecular biology Carbon NDI1 Glucose NAD+ kinase NDE1 Mitochondrial respiration |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1707:199-210 |
| ISSN: | 0005-2728 |
| DOI: | 10.1016/j.bbabio.2004.12.008 |
| Popis: | Cloning, sequence and functional analyses of the Kluyveromyces lactis genes KlNDI1 and KlNDE1 are reported. These genes encode for proteins with high homology to the mitochondrial internal (Ndi1p) and external (Nde1p) alternative NADH:ubiquinone oxidoreductases from Saccharomyces cerevisiae and complement the respective mutations. Analysis of KlNDI1 transcriptional regulation showed that expression of this gene is lower in 2% glucose than in 0.5% glucose or non-fermentable carbon sources. Beta-galactosidase activity values, shown by lacZ fusions of KlNDI1 promoter deletions, suggested that two Adr1p binding sites mediate this carbon source regulation of KlNDI1. The expression of the KlNDE1 gene in S. cerevisiae mutant strains and measurement of respiration with isolated mitochondria showed that the protein encoded by KlNDE1 oxidizes NADPH, this being an important difference with respect to the conventional yeast S. cerevisiae. Moreover, Northern blot experiments using a phosphoglucose isomerase mutant showed that KlNDE1 gene transcription increases with glucose metabolism through the pentose phosphate pathway. |
| Databáze: | OpenAIRE |
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