An Endocannabinoid Signaling System Modulates Anxiety-like Behavior in Male Syrian Hamsters
| Autor: | Anna M. Moise, Giuseppe Astarita, Daniele Piomelli, Andrea G. Hohmann, Sarah A. Eisenstein |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Dominance-Subordination
Male medicine.medical_specialty Cannabinoid receptor Cannabinoid Receptor Modulators medicine.medical_treatment Hamster Oleic Acids Palmitic Acids Depolarization-induced suppression of inhibition Social Environment Article Amidohydrolases chemistry.chemical_compound Piperidines Receptor Cannabinoid CB1 Internal medicine Cricetinae medicine Animals Maze Learning Pharmacology Mesocricetus Brain Anandamide Fear URB597 Endocannabinoid system Amides Aggression Endocrinology nervous system chemistry Ethanolamines Rotarod Performance Test Benzamides Autoradiography Pyrazoles lipids (amino acids peptides and proteins) Cannabinoid Carbamates Rimonabant Arousal psychological phenomena and processes Endocannabinoids Signal Transduction |
| Zdroj: | Moise, AM; Eisenstein, SA; Astarita, G; Piomelli, D; & Hohmann, AG. (2008). An endocannabinoid signaling system modulates anxiety-like behavior in male Syrian hamsters. Psychopharmacology, 200(3), 333-346. doi: 10.1007/s00213-008-1209-5. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/44n526bj |
| DOI: | 10.1007/s00213-008-1209-5. |
| Popis: | Rationale: An endocannabinoid signaling system has not been identified in hamsters. Objective: We examined the existence of an endocannabinoid signaling system in Syrian hamsters using neuroanatomical, biochemical, and behavioral pharmacological approaches. Materials and methods: The distribution of cannabinoid receptors was mapped, and membrane fatty-acid amide hydrolase (FAAH) activity and levels of fatty-acid amides were measured in hamster brain. The impact of cannabinoid CB1receptor blockade and inhibition of FAAH was evaluated in the elevated plus maze, rota-rod test, and models of unconditioned and conditioned social defeat. Results: A characteristic heterogeneous distribution of cannabinoid receptors was detected in hamster brain using [3H]CP55,940 binding and autoradiography. The FAAH inhibitor URB597 inhibited FAAH activity (IC50=12.8 nM) and elevated levels of fatty-acid amides (N-palmitoyl ethanolamine and N-oleoyl ethanolamine) in hamster brain. Anandamide levels were not reliably altered. The cannabinoid agonist WIN55,212-2 (1- 10 mg/kg i.p.) induced CB1-mediated motor ataxia. Blockade of CB1with rimonabant (5 mg/kg i.p.) induced anxiogenic-like behavior in the elevated plus maze. URB597 (0.1-0.3 mg/kg i.p.) induced CB1-mediated anxiolytic-like effects in the elevated plus maze, similar to the benzodiazepine diazepam (2 mg/kg i.p.). Diazepam (2-6 mg/kg i.p.) suppressed the expression, but not the acquisition, of conditioned defeat. By contrast, neither URB597 (0.3-3.0 mg/kg i.p.) nor rimonabant (5 mg/kg i.p.) altered unconditioned or conditioned social defeat or rota-rod performance. Conclusions: Endocannabinoids engage functional CB1receptors in hamster brain to suppress anxiety-like behavior and undergo enzymatic hydrolysis catalyzed by FAAH. Our results further suggest that neither unconditioned nor conditioned social defeat in the Syrian hamster is dependent upon cannabinoid CB1receptor activation. © 2008 Springer-Verlag. |
| Databáze: | OpenAIRE |
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