Vasoactive intestinal peptide administration after stroke in rats enhances neurogenesis and improves neurological function

Autor: Yi-Hua Qian, Chang-hong Zong, Qing-Dong Shi, Ling Chang, Gai-Feng Feng, Yuan-Bo Yang, Jie Yang
Rok vydání: 2015
Předmět:
Doublecortin Domain Proteins
Male
Vascular Endothelial Growth Factor A
medicine.medical_specialty
Doublecortin Protein
Time Factors
Angiogenesis
Neurogenesis
Vasoactive intestinal peptide
Ischemia
Subventricular zone
Antigens
CD34
Cell Count
Rats
Sprague-Dawley
chemistry.chemical_compound
Internal medicine
medicine
Animals
Molecular Biology
biology
Cerebral infarction
General Neuroscience
Neuropeptides
Endothelial Cells
Infarction
Middle Cerebral Artery
medicine.disease
Rats
Doublecortin
Vascular endothelial growth factor
Disease Models
Animal
medicine.anatomical_structure
Endocrinology
Bromodeoxyuridine
nervous system
chemistry
biology.protein
Neurology (clinical)
Nervous System Diseases
Microtubule-Associated Proteins
Neuroscience
hormones
hormone substitutes
and hormone antagonists
Vasoactive Intestinal Peptide
Developmental Biology
Zdroj: Brain Research. 1625:189-197
ISSN: 0006-8993
DOI: 10.1016/j.brainres.2015.09.001
Popis: The aim of this study was to investigate the effects of vasoactive intestinal peptide (VIP) on neurogenesis and neurological function after cerebral ischemia. Rats were intracerebroventricular administered with VIP after a 2h middle cerebral artery occlusion (MCAO) and sacrificed at 7, 14 and 28 days after MCAO. Functional outcome was studied with the modified neurological severity score. The infarct volume was evaluated via histology. Neurogenesis, angiogenesis and the protein expression of vascular endothelial growth factor (VEGF) were measured by immunohistochemistry and Western blotting analysis, respectively. The treatment with VIP significantly reduced the neurological severity score and the infarc volume, and increased the numbers of bromodeoxyuridine (BrdU) immunoreactive cells and doublecortin immunoreactive area in the subventricular zone (SVZ) at 7, 14 and 28 days after ischemia. The cerebral protein levels of VEGF and VEGF expression in the SVZ were also enhanced in VIP-treated rats at 7 days after stroke. VIP treatment obviously increased the number of BrdU positive endothelial cells in the SVZ and density of cerebral microvessels in the ischemic boundary at 28 days after ischemia. Our study suggests that in the ischemic rat brain VIP reduces brain damage and promotes neurogenesis by increasing VEGF. VIP-enhanced neurogenesis is associated with angiogenesis. These changes may contribute to improvement in functional outcome.
Databáze: OpenAIRE
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