Molecular detection and identification of hemoparasites in pampas deer (Ozotoceros bezoarticus Linnaeus, 1758) from the Pantanal Brazil
Autor: | Walfrido Moraes Tomas, Múcio Flávio Barbosa Ribeiro, Renata G.P. Tomich, Ana Cristina R. Lacerda, Élida Mara Leite Rabelo, Paulo André Lima Borges, Aiesca Oliveira Pellegrin, Julia A.G. Silveira |
---|---|
Rok vydání: | 2013 |
Předmět: |
Male
Veterinary medicine biology Ecology Deer Pampas deer Babesia bovis DNA Protozoan biology.organism_classification Microbiology 18S ribosomal RNA Infectious Diseases Insect Science GenBank parasitic diseases Babesia Theileria Animals Female Parasitology Anaplasma Protozoan Infections Animal Nested polymerase chain reaction Brazil |
Zdroj: | Ticks and Tick-borne Diseases. 4:341-345 |
ISSN: | 1877-959X |
Popis: | Hemoparasites were surveyed in 60 free-living pampas deer Ozotoceros bezoarticus from the central area of the Pantanal, known as Nhecolândia, State of Mato Grosso do Sul, Brazil, through the analysis of nested PCR assays and nucleotide sequencing. Blood samples were tested for Babesia/Theileria, Anaplasma spp., and Trypanosoma spp. using nPCR assays and sequencing of the 18S rRNA, msp4, ITS, and cathepsin L genes. The identity of each sequence was confirmed by comparison with sequences from GenBank using BLAST software. Forty-six (77%) pampas deer were positive for at least one hemoparasite, according to PCR assays. Co-infection occurred in 13 (22%) animals. Based on the sequencing results, 29 (48%) tested positive for A. marginale. Babesia/Theileria were detected in 23 (38%) samples, and according to the sequencing results 52% (12/23) of the samples were similar to T. cervi, 13% (3/23) were similar to Babesia bovis, and 9% (2/23) were similar to B. bigemina. No samples were amplified with the primers for T. vivax, while 11 (18%) were amplified with the ITS primers for T. evansi. The results showed pampas deer to be co-infected with several hemoparasites, including species that may cause serious disease in cattle. Pampas deer is an endangered species in Brazil, and the consequences of these infections to their health are poorly understood. |
Databáze: | OpenAIRE |
Externí odkaz: |