The detection of serum anti-p53 antibodies from patients with gastric carcinoma in China
| Autor: | Tian Qiu, Panyu Hua, Li-Li Ye, Lin-Lin Qiu, Hui-Zheng Bao, Yong-Cai Wang, Li Wang |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Adult
Male China Cancer Research Pathology medicine.medical_specialty Adolescent Antibodies Neoplasm Blotting Western Enzyme-Linked Immunosorbent Assay Gastric carcinoma Biology law.invention Carcinoembryonic antigen Peptide Library Stomach Neoplasms law Biomarkers Tumor medicine Humans P53 antibodies Aged Autoantibodies Neoplasm Staging Tumor size Case-control study Middle Aged Carcinoembryonic Antigen Blot Oncology Case-Control Studies Recombinant DNA biology.protein Female Tumor Suppressor Protein p53 Antibody |
| Zdroj: | Cancer Detection and Prevention. 31:45-49 |
| ISSN: | 0361-090X |
| Popis: | Background : Gastric carcinoma is one of the most frequently occurring cancers. The aim of this research was to increase the detection efficiency of anti-p53 antibodies in the sera of patients with gastric carcinoma and to improve the diagnosis for patients with gastric carcinoma. Methods : We prepared phage-displayed peptide DO7 and established an enzyme-linked immunosorbent assay method to detect the anti-p53 antibodies. We detected the anti-p53 antibodies of 61 patients with gastric carcinoma using the method and our previous ELISA method assisted by the recombinant wild-type human p53 protein to detect the anti-p53 antibodies. We studied the correlation between the anti-p53 antibodies and the clinicopathological data including sex, age, carcinoembryonic antigen, tumor size, tumor TNM staging, and lymph-node status. Results : The anti-p53 antibodies positive rate for patients with gastric carcinoma was increased (31.1%, 19/61) through the combination of p53-ELISA and phage-ELISA. We found that the positive anti-p53 antibodies correlated significantly with tumor size ( P =0.047). The combination of the anti-p53 antibodies and carcinoembryonic antigen could improve the diagnosis for patients with gastric carcinoma. Conclusions : This approach indicated an increased anti-p53 antibodies positive rate for patients with gastric carcinoma and provided a useful marker for clinical diagnosis for patients with gastric carcinoma. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect