Suppression of interleukin-2 and interleukin-2 receptor expression in Jurkat cells stably expressing the human immunodeficiency virus Tat protein
| Autor: | Michael M. Lederman, Daniel L. Georges, Thomas M. Williams, Scott F. Purvis |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Interleukin 2
Gene Expression Regulation Viral viruses Receptor expression T cell Immunology Biology Transfection Jurkat cells Cell Line Suppression Genetic medicine Humans Phytohemagglutinins Receptor Messenger RNA Receptors Interleukin-2 Virology Molecular biology medicine.anatomical_structure Cell culture Gene Products tat Interleukin-2 Tetradecanoylphorbol Acetate medicine.drug |
| Zdroj: | Cellular immunology. 144(1) |
| ISSN: | 0008-8749 |
| Popis: | The Jurkat T cell line was stably transfected with an Epstein-Barr virus-based episomal replicon designed to express high levels of the HIV-1 Tat protein. After selection in hygromycin B, high-level Tat activity was detected in 3 of 18 transfected cell lines. After stimulation with phytohemag-glutinin (PHA) and phorbol myristate acetate (PMA), Tat transfectants with high Tat expression showed diminished expression of interleukin-2 (IL-2) and the interleukin-2 receptor a chain (IL-2R) when compared to untransfected Jurkat cells or Jurkat cell lines transfected with the parent control plasmid. Sublines derived from the high-level Tat transfectants with reduced Tat activity showed normalization of PHA/PMA-induced IL-2 expression. Northern analysis showed diminished expression of IL-2 and IL-2R mRNA in the stimulated Tat transfectants. Inhibition of IL-2 and IL-2R expression by the HIV-1 Tat protein may contribute to the immune suppression that characterizes HIV-1 infection. |
| Databáze: | OpenAIRE |
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