Development of an ic-ELISA and immunochromatographic strip for detection of sparfloxacin in honey
| Autor: | Guochao Jia, Aimin Zhi, Jingjing Li, Hui Nie, Lianzhong Ai |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.drug_class Agriculture (General) 030106 microbiology Immunology honey sparfloxacin Monoclonal antibody 01 natural sciences S1-972 03 medical and health sciences medicine Chemistry 010401 analytical chemistry ic-elisa RC581-607 ica strip Molecular biology 0104 chemical sciences Sparfloxacin monoclonal antibody Immunologic diseases. Allergy Ic elisa Agronomy and Crop Science Food Science medicine.drug |
| Zdroj: | Food and Agricultural Immunology, Vol 29, Iss 1, Pp 147-158 (2018) |
| ISSN: | 1465-3443 0954-0105 |
| DOI: | 10.1080/09540105.2017.1364706 |
| Popis: | In this study, a sensitive monoclonal antibody (mAb) 3D5 against sparfloxacin (SPFX) was generated. Based on the mAb 3D5, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method was developed for the detection of SPFX in honey. The half maximal inhibitory concentration and limit of detection for the ic-ELISA method were 0.12 and 0.02 ng/mL, respectively. The experiments showed a negligible cross-reaction with other drugs. The average recovery rates for spiked SPFX honey extracts ranged from 90% to 101%, indicating an accepted accuracy. Furthermore, a lateral-flow immunochromatographic assay strip method with a cut-off value of 2 ng/mL was developed. Therefore, both of the developed methods are suitable for future use as rapid screening methods to detect and control the content of SPFX residues in honey samples. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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