Toxic effect of acrylamide on the development of hippocampal neurons of weaning rats
| Autor: | Li Luo, Meng-meng Zhao, Ziting Gu, Yuxin Ma, Jing Liu, Xi-xia Li, Sheng-min Lai |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty hippocampus synaptophysin Developmental toxicity neurons growth associated protein 43 lcsh:RC346-429 weaning rats 03 medical and health sciences symbols.namesake chemistry.chemical_compound 0302 clinical medicine Developmental Neuroscience Internal medicine medicine developmental toxicity Weaning nerve regeneration acrylamide dentate gyrus protein developmental neurobiology neural regeneration Gap-43 protein lcsh:Neurology. Diseases of the nervous system biology Dentate gyrus Neurotoxicity medicine.disease 030104 developmental biology Endocrinology nervous system chemistry Acrylamide Nissl body symbols Synaptophysin biology.protein 030217 neurology & neurosurgery Research Article |
| Zdroj: | Neural Regeneration Research Neural Regeneration Research, Vol 12, Iss 10, Pp 1648-1654 (2017) |
| ISSN: | 1673-5374 |
| DOI: | 10.4103/1673-5374.217345 |
| Popis: | Although numerous studies have examined the neurotoxicity of acrylamide in adult animals, the effects on neuronal development in the embryonic and lactational periods are largely unknown. Thus, we examined the toxicity of acrylamide on neuronal development in the hippocampus of fetal rats during pregnancy. Sprague-Dawley rats were mated with male rats at a 1:1 ratio. Rats were administered 0, 5, 10 or 20 mg/kg acrylamide intragastrically from embryonic days 6–21. The gait scores were examined in pregnant rats in each group to analyze maternal toxicity. Eight weaning rats from each group were also euthanized on postnatal day 21 for follow-up studies. Nissl staining was used to observe histological change in the hippocampus. Immunohistochemistry was conducted to observe the condition of neurites, including dendrites and axons. Western blot assay was used to measure the expression levels of the specific nerve axon membrane protein, growth associated protein 43, and the presynaptic vesicle membrane specific protein, synaptophysin. The gait scores of gravid rats significantly increased, suggesting that acrylamide induced maternal motor dysfunction. The number of neurons, as well as expression of growth associated protein 43 and synaptophysin, was reduced with increasing acrylamide dose in postnatal day 21 weaning rats. These data suggest that acrylamide exerts dose-dependent toxic effects on the growth and development of hippocampal neurons of weaning rats. |
| Databáze: | OpenAIRE |
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