High alpha-methylacyl-CoA racemase (AMACR) is associated with ERG expression and with adverse clinical outcome in patients with localized prostate cancer

Autor: Mohammed Alshalalfa, Tarek A. Bismar, Adrian Box, Bryan Donnelly, Samar A Hegazy
Rok vydání: 2016
Předmět:
0301 basic medicine
Oncology
Biochemical recurrence
Adult
Male
Pathology
medicine.medical_specialty
Surgical margin
genetic structures
Oncogene Proteins
Fusion
Blotting
Western
Racemases and Epimerases
Kaplan-Meier Estimate
Biology
Alpha-methylacyl-CoA racemase
03 medical and health sciences
Prostate cancer
0302 clinical medicine
Transcriptional Regulator ERG
Internal medicine
Cell Line
Tumor
medicine
Biomarkers
Tumor
Humans
Aged
Aged
80 and over
Prostatectomy
Reverse Transcriptase Polymerase Chain Reaction
breakpoint cluster region
Cancer
Prostatic Neoplasms
General Medicine
Gene rearrangement
Middle Aged
medicine.disease
Prognosis
Immunohistochemistry
Gene Expression Regulation
Neoplastic
030104 developmental biology
Treatment Outcome
030220 oncology & carcinogenesis
RNA Interference
sense organs
Neoplasm Grading
Erg
Zdroj: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 37(9)
ISSN: 1423-0380
Popis: Alpha-methylacyl-CoA racemase (AMACR) is a well-characterized marker extensively utilized in prostate cancer (PCA) diagnosis. However, the prognostic value of AMACR expression and its relation to TMPRSS2-ERG gene rearrangement as one of the most common molecular alterations in PCA is not fully explored. AMACR expression was investigated in a cohort of 218 men with localized PCA treated by radical prostatectomy and correlated with ERG and various clinical and pathological parameters. In vitro studies assessed AMACR changes to ERG knockdown and other related genes. In addition, bioinformatics validated the significance of AMACR/ERG expression and assessed relevant genetic signatures in relation to AMACR/ERG expression. AMACR expression was significantly associated with disease progression and with ERG (p ∼0). Seventeen percent of cancer foci showed negative/weak AMACR expression while being ERG positive. High AMACR expression was significantly associated with positive surgical margins (p = 0.01), specifically in tumors with lower Gleason score
Databáze: OpenAIRE
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