Plasma Peptidome as a Source of Biomarkers for Diagnosis of Cholangiocarcinoma
| Autor: | Veerachai Thitapakorn, Kesara Na-Bangchang, Vithoon Viyanant, Narumon Phaonakrop, Sittiruk Roytrakul, Kanawut Kotawong |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine Gene isoform Cancer Research Casein Kinase II Subunit Alpha Epidemiology Protein subunit Opisthorchiasis Cholangiocarcinoma 03 medical and health sciences chemistry.chemical_compound Risk Factors Tandem Mass Spectrometry parasitic diseases Biomarkers Tumor Animals Humans Phosphatidylinositol Opisthorchis viverrini Transcription factor Aged biology Opisthorchis Public Health Environmental and Occupational Health Middle Aged Prognosis Thailand biology.organism_classification Peptide Fragments Bile Ducts Intrahepatic 030104 developmental biology Bile Duct Neoplasms Oncology chemistry Case-Control Studies Cancer research Biomarker (medicine) Female Signal transduction Chromatography Liquid Follow-Up Studies |
| Zdroj: | Asian Pacific Journal of Cancer Prevention. 17:1163-1168 |
| ISSN: | 1513-7368 |
| Popis: | Cholangiocarcinoma (CCA) is the bile duct cancer which constitutes one of the important public health problems in Thailand with high mortality rate, especially in the Opisthorchis viverrini (a parasite risk factor for CCA) endemic area of the northeastern region of the country. This study aimed to identify potential biomarkers from the plasma peptidome by CCA patients. Peptides were isolated using 10 kDa cut-off filter column and the flow-through was then used as a peptidome for LC-MS/MS analysis. A total of 209 peptides were obtained. Among these, 15 peptides were concerned with signaling pathways and 12 related to metabolic, regulatory, and biosynthesis of secondary metabolite pathways. Five exclusive peptides were identified as potential biomarkers, i.e. ETS domain-containing transcription factor ERF (P50548), KIAA0220 (Q92617), phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform isoform 1 (P42338), LP2209 (Q6XYC0), and casein kinase II subunit alpha (P19784). Three of these biomarkers are signaling related molecules. A combination of these biomarkers for CCA diagnosis is proposed. |
| Databáze: | OpenAIRE |
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