The effect of the anaesthetic agent isoflurane on the rate of neutrophil apoptosisin vitro
| Autor: | M. Halligan, R. Tyther, George D. Shorten, N F Fanning, Henry Paul Redmond, Jiang Huai Wang |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Adult
Time Factors Neutrophils Apoptosis In Vitro Techniques Pharmacology chemistry.chemical_compound Annexin medicine Humans Propidium iodide Cells Cultured Isoflurane business.industry General Medicine Venous blood Flow Cytometry In vitro Fresh gas flow chemistry Anesthesia Anesthetics Inhalation Halothane business medicine.drug |
| Zdroj: | Irish Journal of Medical Science. 170 |
| ISSN: | 1863-4362 0021-1265 |
| DOI: | 10.1007/bf03167720 |
| Popis: | Volatile anaesthetic agents influence neutrophil function, and potentially, the inflammatory response to surgery.The objective of this study was to determine the effect of isoflurane (1-4%) on human polymorphonuclear neutrophil apoptosis in vitro.Venous blood from 12 healthy volunteers was exposed to 0, 1, and 4% isoflurane delivered via a 14G Wallace flexihub internal jugular cannula, at a fresh gas flow of 0.51/min for 5 minutes. Isolated neutrophils were assessed for apoptosis at 1, 12, and 24 hours in culture using dual staining with annexin V-FITC and propidium iodide (Annexin-V FITC assay). Data were analysed using paired, one-tailed Student's t-tests. p0.05 was considered significant.At 1 hour apoptosis was inhibited in the 1% (5.1 [6.8]%; p=0.017) and 4% (4.8 [4.5]%; p=0.008) isoflurane groups compared to control (11.3 [6.9]%). At 12 and 24 hours, a dose-dependent inhibition of apoptosis was demonstrated, i.e. 4%1%0%.Human neutrophil apoptosis is inhibited in a concentration-dependent manner in vitro by isoflurane in clinical concentrations. |
| Databáze: | OpenAIRE |
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