High resolution FESEM and TEM reveal bacterial spore attachment
| Autor: | G.T. Tortora, Barbara J. Panessa-Warren, John B. Warren |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Materials science
Scanning electron microscope Analytical chemistry Endospore Bacterial Adhesion law.invention Bacillus cereus Microscopy Electron Transmission law Microscopy Humans Trypsin Instrumentation Spores Bacterial Clostridioides difficile fungi Spore Chloramphenicol Transmission electron microscopy Biophysics Ultrastructure Microscopy Electron Scanning Bacterial spore Glass Electron microscope Caco-2 Cells |
| Zdroj: | Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada. 13(4) |
| ISSN: | 1431-9276 |
| Popis: | Transmission electron microscopy (TEM) studies in the 1960s and early 1970s using conventional thin section and freeze fracture methodologies revealed ultrastructural bacterial spore appendages. However, the limited technology at that time necessitated the time-consuming process of imaging serial sections and reconstructing each structure. Consequently, the distribution and function of these appendages and their possible role in colonization or pathogenesis remained unknown. By combining high resolution field emission electron microscopy with TEM images of identical bacterial spore preparations, we have been able to obtain images of intact and sectioned Bacillus and Clostridial spores to clearly visualize the appearance, distribution, resistance (to trypsin, chloramphenicol, and heat), and participation of these structures to facilitate attachment of the spores to glass, agar, and human cell substrates. Current user-friendly commercial field emission scanning electron microscopes (FESEMs), permit high resolution imaging, with high brightness guns at lower accelerating voltages for beam sensitive intact biological samples, providing surface images at TEM magnifications for making direct comparisons. For the first time, attachment structures used by pathogenic, environmental, and thermophile bacterial spores could be readily visualized on intact spores to reveal how specific appendages and outer spore coats participated in spore attachment, colonization, and invasion. |
| Databáze: | OpenAIRE |
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