Advantage of 16S rRNA amplicon sequencing in Helicobacter pylori diagnosis
| Autor: | Yoshio Yamaoka, Takashi Matsumoto, Dashdorj Azzaya, Boldbaatar Gantuya, Duger Davaadorj, Khasag Oyuntsetseg, Tomohisa Uchida, Batsaikhan Saruuljavkhlan, Nyamdorj Oyunbileg, Hashem B. El–Serag |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male medicine.medical_specialty Concordance Rapid urease test Sensitivity and Specificity Gastroenterology Article Helicobacter Infections Serology 03 medical and health sciences 0302 clinical medicine RNA Ribosomal 16S Internal medicine medicine Humans Helicobacter pylori Receiver operating characteristic biology business.industry Mongolia General Medicine 16S ribosomal RNA biology.organism_classification Infectious Diseases 030220 oncology & carcinogenesis Biomarker (medicine) Female 030211 gastroenterology & hepatology Histopathology business |
| Zdroj: | Helicobacter |
| ISSN: | 1523-5378 1083-4389 |
| DOI: | 10.1111/hel.12790 |
| Popis: | BACKGROUND 16S rRNA amplicon sequencing is an accurate method of detecting microbial infection without culture. It is unclear if sequencing has additional benefits over routine diagnostic methods for Helicobacter pylori testing. METHODS We enrolled Mongolian volunteers with dyspepsia. Using routine diagnostic methods, positive H. pylori was defined as positive results on histology/immunohistochemistry, culture, rapid urease test, or serology; negative H. pylori was defined by negative results from all these tests. We performed 16S rRNA sequencing on gastric biopsy specimens and calculated cutoffs for operational taxonomic units (OTUs) and relative abundance (RA) to define positive results using ROC curves. RESULTS We examined 161 individuals with a mean age of 43.6 years, and 64.6% were women. Using routine diagnostic methods, 122 (75.8%) participants were H. pylori positive, the sensitivity and specificity for 16S rRNA sequencing were 94.3% and 82.1% or 93.4% and 82.1% when cutoff values were set to 1113 (OTU number) or 4.4% RA, respectively (both p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text