ATP-activated cationic and anionic conductances in cultured rat hippocampal neurons
| Autor: | Max R. Bennett, Chandra Balachandran |
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| Rok vydání: | 1996 |
| Předmět: |
Agonist
Patch-Clamp Techniques medicine.drug_class Suramin Hippocampal formation Biology Hippocampus Ion Channels Membrane Potentials Adenosine Triphosphate medicine Animals heterocyclic compounds Rats Wistar Reversal potential Cells Cultured Neurons Receptors Purinergic P2 General Neuroscience Purinergic receptor Cationic polymerization Adenosine Rats Electrophysiology Biophysics Neuroscience medicine.drug |
| Zdroj: | Neuroscience Letters. 204:73-76 |
| ISSN: | 0304-3940 |
| Popis: | The effects of adenosine 5′-triphosphate (ATP) on voltage-clamped and dissociated rat hippocampal neurons was investigated. Over 30% of neurons possessed ATP-activated inward currents at a holding potential of −70 mV. The ED50 for these currents was 150 μM. At this concentration they were blocked by suramin (1 mM), indicating the involvement of P2-purinoceptors. The P2 purinoceptor agonist potency was 2-methylthio ATP > ATP > ADP > α,β-methylene ATP, thus identifying these purinoceptors as belonging to the P2x subclass. The reversal potential for the ATP-activated currents was −45 ± 8 mV. Ion substitution experiments showed that the permeability ratio for K+/Na+/Cs+/Cl−, was 18:3:2:1, according to the Goldman-Hodgkin-Katz equation, so that ATP activates cationic and anionic conductances in hippocampal neurons. |
| Databáze: | OpenAIRE |
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