Interleukin-2-dependent augmentation of the anti-TNP antibody production by sodium butyrate in cultured murine splenic B cells
| Autor: | Masami Fujiwara, Toshiko Adachi, Eiichi Gohda, Yumiko Kishiro, Itaru Yamamoto |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Interleukin 2
Lipopolysaccharides medicine.medical_specialty medicine.medical_treatment Immunology Histamine Antagonists Butyrate Biology chemistry.chemical_compound Mice Antigen Internal medicine medicine Splenocyte Animals B cell Cells Cultured Pharmacology B-Lymphocytes Mice Inbred BALB C Sodium butyrate Flow Cytometry Molecular biology Endotoxins Butyrates medicine.anatomical_structure Cytokine Endocrinology chemistry Antibody Formation biology.protein Butyric Acid Interleukin-2 Female Antibody Spleen medicine.drug |
| Zdroj: | International journal of immunopharmacology. 19(6) |
| ISSN: | 0192-0561 |
| Popis: | Previously we found that sodium butyrate (NaBu) markedly enhanced production of the antibody specific for a T-cell-dependent antigen, sheep red blood cells (SRBC) in murine splenocytes (Kishiro, Y., Ueda, K., Fujiwara, M. and Yamamoto, I., Jpn J. Phamacol., 1994 66, 369-376. To gain a better understanding of the target cells for NaBu's action on antibody responses, we have utilized the T-cell-independent antigen, trinitrophenyl-lypopolysaccharide (TNP-LPS) as a stimulant and have examined an effect of NaBu on the anti-TNP antibody production in vitro. NaBu markedly increased the anti-TNP plaque-forming cell (PFC) responses in murine whole splenocytes, but not in murine splenic B cells. Addition of T-cells or the concanavalin A supernatant (CAS) from murine splenocytes to the B cell cultures completely restored the enhancing effect of NaBu. This effect of CAS was totally blocked by an anti-interleukin (IL)-2 antibody and partially by an anti-IL-1 beta or anti-IL-4 antibody. The full enhancing effect of NaBu was also detected when IL-2 was added to the B cell cultures, while IL-2 alone had no stimulatory effect on the control PFC response. IL-1 beta alone significantly stimulated the antibody production and adding NaBu to this IL-1 beta-supplemented culture caused a further increase. Neither IL-4 alone nor NaBu plus IL-4 had any effect on the PFC response. NaBu did not affect the expression of the IL-2 receptor alpha- and beta-chains in B cells stimulated with TNP-LPS. These results suggest that NaBu is an agent that promotes B cell differentiation in vitro in an IL-2-dependent manner. |
| Databáze: | OpenAIRE |
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