Expression of Semaphorin 4F in neurons and brain oligodendrocytes and the regulation of oligodendrocyte precursor migration in the optic nerve
| Autor: | Esther Pérez-Martínez, Ferran Burgaya, Eduardo Soriano, Ana Bribián, Beatriz G. Armendáriz, Albert Martínez, Fernando de Castro |
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| Rok vydání: | 2012 |
| Předmět: |
Cellular differentiation
Nerve Tissue Proteins Biology Hippocampus Cell Line Mice Cellular and Molecular Neuroscience Glutamatergic Neural Stem Cells Semaphorin Cell Movement medicine Animals Humans Molecular Biology Cells Cultured Neurons Brain Gene Expression Regulation Developmental Membrane Proteins Cell Differentiation Optic Nerve Cell Biology Neural stem cell Transmembrane protein Oligodendrocyte Oligodendroglia medicine.anatomical_structure Optic nerve Neural development Neuroscience |
| Zdroj: | Molecular and Cellular Neuroscience. 49:54-67 |
| ISSN: | 1044-7431 |
| Popis: | Semaphorins are secreted or membrane-anchored proteins that play critical roles in neural development and adult brain plasticity. Sema4F is a transmembrane semaphorin found on glutamatergic synapses, in which it is attached to the PSD-95-scaffolding protein. Here we further examined the expression of Sema4F by raising specific antibodies. We show that Sema4F protein is widely expressed by neurons during neural development and in the adult brain. We also demonstrate a preferential localization of this protein in postsynaptic dendrites. Moreover, Sema4F is expressed not only by neurons but also by oligodendrocyte precursors in the optic nerve and along the migratory pathways of oligodendroglial cells, and also by subsets of postnatal oligodendroglial cells in the brain. Finally, in vitro experiments demonstrate that endogenous Sema4F expressed by brain cells of oligodendroglial lineage regulates the outgrowth migration of oligodendrocyte precursors and promotes their differentiation. The present data extend our knowledge about the expression of Sema4F and uncover a novel function in the control of oligodendrocyte precursor migration in the developing brain. |
| Databáze: | OpenAIRE |
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