Ccne1 Overexpression Causes Chromosome Instability in Liver Cells and Liver Tumor Development in Mice

Autor: Bart van de Sluis, Grace G. Nelson, Sameh A. Youssef, Erin Hurley, Raul O. Fierro Velasco, Ines Sturmlechner, Alain de Bruin, Jazeel F. Limzerwala, Janine H. van Ree, Gregory J. Gores, Daniel R. O'Brien, Erik Jan van Deursen, Lewis R. Roberts, Floris Foijer, Hilda van den Bos, Jean Pierre A. Kocher, Steve F. Bronk, Cheng Zhang, Khaled Aziz, Hu Li, Diana C.J. Spierings, Karthik B. Jeganathan, Jan M. van Deursen
Přispěvatelé: Stem Cell Aging Leukemia and Lymphoma (SALL), Damage and Repair in Cancer Development and Cancer Treatment (DARE), Center for Liver, Digestive and Metabolic Diseases (CLDM), Restoring Organ Function by Means of Regenerative Medicine (REGENERATE), dPB RMSC, LS Pathobiologie
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Cyclin E
KINASE-INDEPENDENT FUNCTION
Aneuploidy
medicine.disease_cause
Mice
Liver Neoplasms
Experimental
0302 clinical medicine
Parvovirinae
Chromosome instability
CYCLIN-E
HBV
OXIDATIVE STRESS
MUTATION
Cyclin
Oncogene Proteins
Liver Neoplasms
Gastroenterology
Dependovirus
Chromosome Structures
Liver
Chromosome Integrity
AAV2
S Phase Cell Cycle Checkpoints
030211 gastroenterology & hepatology
DNA Replication
EXPRESSION
Hepatocarcinogenesis
Carcinoma
Hepatocellular
Biology
Article
Adenoma
Liver Cell
Parvoviridae Infections
Polyploidy
03 medical and health sciences
MOVEMENT
Hepatitis B
Chronic
COMPENSATORY PROLIFERATION
Chromosomal Instability
medicine
TUMORIGENESIS
Animals
P53
Hepatology
Fibroblasts
HCCS
medicine.disease
CENTROSOMES
Cyclin E1
030104 developmental biology
Cancer cell
Hepatocytes
Cancer research
Carcinogenesis
DNA Damage
Zdroj: Gastroenterology, 157(1). W B SAUNDERS CO-ELSEVIER INC
Gastroenterology, 157(1), 210. W.B. Saunders Ltd
Gastroenterology
ISSN: 0016-5085
Popis: BACKGROUND & AIMS: The CCNE1 locus, which encodes cyclin E1, is amplified in many types of cancer cells and is activated in hepatocellular carcinomas (HCCs) from patients infected with hepatitis B virus or adeno-associated virus type 2, due to integration of the virus nearby. We investigated cell cycle and oncogenic effects of cyclin E1 overexpression in tissues of mice.METHODS: We generated mice with doxycycline-inducible expression of Ccne1 (Ccne1T mice) and activated overexpression of cyclin E1 from age 3 weeks onwards. At 14 months of age, livers were collected from mice that overexpress cyclin E1 and non-transgenic mice (controls) and analyzed for tumor burden and by histology. Mouse embryonic fibroblasts (MEFs) and hepatocytes from Ccne1T and control mice were analyzed to determine the extent to which cyclin E1 overexpression perturbs S-phase entry, DNA replication, and numbers and structures of chromosomes. Tissues from 4-month-old Ccne1T and control mice (at that age were free of tumors) were analyzed for chromosome alterations, to investigate the mechanisms by which cyclin E1 predisposes hepatocytes to transformation.RESULTS: Ccne1T mice developed more hepatocellular adenomas and HCCs than control mice. Tumors developed only in livers of Ccne1T mice, despite high levels of cyclin E1 in other tissues. Ccne1T MEFs had defects that promoted chromosome missegregation and aneuploidy, including incomplete replication of DNA, centrosome amplification, and formation of non-perpendicular mitotic spindles. Whereas Ccne1T mice accumulated near-diploid aneuploid cells in multiple tissues and organs, polyploidization was observed only in hepatocytes, with losses and gains of whole chromosomes, DNA damage, and oxidative stress.CONCLUSIONS: Livers, but not other tissues of mice with inducible overexpression of cyclin E1, develop tumors. More hepatocytes from the cyclin E1-overexpressing mice were polyploid than from control mice, and had losses or gains of whole chromosomes, DNA damage, and oxidative stress - all of these have been observed in human HCC cells. The increased risk of HCC in patients with hepatitis B virus or adeno-associated virus type 2 infection might involve activation of cyclin E1 and its effects on chromosomes and genomes of liver cells.
Databáze: OpenAIRE
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