The influence of non-cytotoxic concentrations of the herbicide 2,4-dichlorophenoxyacetic acid on the DNA synthesis in cultured vertebrate cells
| Autor: | Dieter Preiss, Klaus Goerttler, Dieter Haag |
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| Rok vydání: | 1975 |
| Předmět: |
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4-Dichlorophenoxyacetic acid Health Toxicology and Mutagenesis Cellular differentiation Chick Embryo Biology Toxicology Fluorescence chemistry.chemical_compound Cytotoxic T cell Animals Cells Cultured Cell Nucleus DNA synthesis Dose-Response Relationship Drug Staining and Labeling Muscles Cell Differentiation General Medicine DNA Nuclear matrix Molecular biology Chromatin chemistry Growth inhibition 2 4-Dichlorophenoxyacetic Acid |
| Zdroj: | Archives of toxicology. 33(2) |
| ISSN: | 0340-5761 |
| Popis: | The influence of the herbicide 2,4-Dichlorophenoxyacetic acid on the growth of cultured chicken muscle cells was studied by observing morphological changes and by cytophotometric measurement of the DNA content of individual cells. DNA-synthesis curves were derived from the distribution of DNA values. No marked effects were observed at a concentration of 60 mug per liter. In contrast, doses of 600 mug per liter caused a loss in cell differentiation consisting of a partial lack of polar orientation, diminished formation of fibers, and an increase of the N/C ratio. The nuclear matrix of desoxyribonucleoprotein (DNP) changed to a more heteropycnotic structure. Isolated cell necroses were found after 44 hrs of exposure. When compared to the controls, the relative DNA synthesis time was found to be prolonged 1.5-fold. The number of cells in the G2 phase decreased during 44 hrs of exposure. It is concluded from these results that the dosedependent growth inhibition of 2,4-D on vertebrate cells in culture takes effect during DNA synthesis. The morphological changes of the nuclear structure suggests that 2,4-D directly affects the template activity of the DNP matrix. |
| Databáze: | OpenAIRE |
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