Anti-inflammatory function of ginsenoside Rg1 on alcoholic hepatitis through glucocorticoid receptor related nuclear factor-kappa B pathway
| Autor: | Mei-Jin Zhang, Wei Guining, Zhao Zhang, Chu Shifeng, Jianping Li, Yan Gao, Yueting Li, Jin-Feng Hu, Nai-Hong Chen, Jing-Wei Li, Cong-Yuan Xia, Yang Heng |
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| Rok vydání: | 2015 |
| Předmět: |
Male
medicine.medical_specialty Ginsenosides medicine.drug_class Anti-Inflammatory Agents Alcoholic hepatitis Panax Protective Agents Plant Roots Anti-inflammatory Proinflammatory cytokine Ginseng Glucocorticoid receptor Receptors Glucocorticoid Western blot Internal medicine Drug Discovery medicine Animals Aspartate Aminotransferases Dexamethasone Triglycerides Pharmacology biology medicine.diagnostic_test L-Lactate Dehydrogenase business.industry Hepatitis Alcoholic NF-kappa B Alanine Transaminase medicine.disease Mice Inbred C57BL Endocrinology Cholesterol Alanine transaminase Liver biology.protein Cytokines business medicine.drug |
| Zdroj: | Journal of ethnopharmacology. 173 |
| ISSN: | 1872-7573 |
| Popis: | Ethnopharmacological relevance Ginseng is the dried root of Panax ginseng C.A. Mayer. Since ancient times, ginseng has been used as one kind of treatment drug or tonic in China and even other eastern countries like Korea and Japan. Pharmacological active chemical ingredients and its extract of ginseng are a mixture of triterpenoid saponins, collectively called ginsenosides. Among them, ginsenoside Rg1 is the most pharmacological active one. Aim of the study Based on prior experimental results and the understanding of alcoholic hepatitis, the major aim of this study is to investigate whether Rg1 is beneficial in a rodent model mimic alcoholic hepatic injury associated with binge drinking and explore the underlying possible mechanisms. Materials and methods C57BL/6 mice were given oral consumption of 6 g/kg alcohol 1 h after treated with Rg1 (10, 20 and 40 mg/kg) or dexamethasone (1 mg/kg) for 9 consecutive days. Biochemical analyses were performed and liver fragments were processed for microscopy, immunohistochemistry and western blot analysis. Results According to our data, Rg1 treatment significantly reversed the high mortality rate induced by alcohol consumption and also alleviated liver impairment as evidenced by the decrease of serum parameters. Meanwhile, histological and ultrastructural analysis of alcoholic groups showed hepatocellular impairment but restored in Rg1-treated groups. Overproductive inflammatory cytokines were also suppressed by Rg1 in alcohol-intoxicated mouse livers. In addition, changes of GR related NF-κB pathway, including phospho-IκB-α, were also modulated to normal levels. Conclusion This study demonstrates that Rg1 might promote GR mediating the repression of NF-κB and inhibit the inflammatory reactions in alcoholic hepatitis. |
| Databáze: | OpenAIRE |
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