Two stage heterotrophy/photoinduction culture of Scenedesmus incrassatulus : potential for lutein production
| Autor: | Liliana Flórez-Miranda, Orlando Melchy-Antonio, Cesar Mateo Flores Ortiz, Fernando Martínez-Jerónimo, Rosa Olivia Cañizares-Villanueva |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Lutein Time Factors Light Nitrogen Heterotroph Biomass Photobioreactor Bioengineering Biology 01 natural sciences Applied Microbiology and Biotechnology Photobioreactors 03 medical and health sciences chemistry.chemical_compound Bioreactors 010608 biotechnology Botany Bioreactor Yeast extract Autotroph Food science chemistry.chemical_classification Autotrophic Processes Temperature food and beverages Heterotrophic Processes Vitamins General Medicine Carbon Culture Media Kinetics Glucose 030104 developmental biology chemistry Xanthophyll Fermentation Scenedesmus Biotechnology |
| Zdroj: | Journal of Biotechnology. 262:67-74 |
| ISSN: | 0168-1656 |
| DOI: | 10.1016/j.jbiotec.2017.09.002 |
| Popis: | A biomass production process including two stages, heterotrophy/photoinduction (TSHP), was developed to improve biomass and lutein production by the green microalgae Scenedesmus incrassatulus. To determine the effects of different nitrogen sources (yeast extract and urea) and temperature in the heterotrophic stage, experiments using shake flask cultures with glucose as the carbon source were carried out. The highest biomass productivity and specific pigment concentrations were reached using urea+vitamins (U+V) at 30°C. The first stage of the TSHP process was done in a 6L bioreactor, and the inductions in a 3L airlift photobioreactor. At the end of the heterotrophic stage, S. incrassatulus achieved the maximal biomass concentration, increasing from 7.22gL-1 to 17.98gL-1 with an increase in initial glucose concentration from 10.6gL-1 to 30.3gL-1. However, the higher initial glucose concentration resulted in a lower specific growth rate (μ) and lower cell yield (Yx/s), possibly due to substrate inhibition. After 24h of photoinduction, lutein content in S. incrassatulus biomass was 7 times higher than that obtained at the end of heterotrophic cultivation, and the lutein productivity was 1.6 times higher compared with autotrophic culture of this microalga. Hence, the two-stage heterotrophy/photoinduction culture is an effective strategy for high cell density and lutein production in S. incrassatulus. |
| Databáze: | OpenAIRE |
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