T Cell Epitopes in Japanese Cedar (Cryptomeria japonica) Pollen Allergens: Choice of Major T Cell Epitopes in Cry j 1 and Cry j 2 Toward Design of the Peptide-Based Immunotherapeutics for the Management of Japanese Cedar Pollinosis
| Autor: | T, Sone, K, Morikubo, M, Miyahara, N, Komiyama, K, Shimizu, H, Tsunoo, K, Kino |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
Male T-Lymphocytes Molecular Sequence Data Immunology Epitopes T-Lymphocyte Lymphocyte Activation Cell Line Trees HLA Antigens Consensus Sequence Humans Immunology and Allergy Amino Acid Sequence Plant Proteins Antigen Presentation Base Sequence Rhinitis Allergic Seasonal Allergens Antigens Plant Immunoglobulin E Middle Aged Pollen Female Binding Sites Antibody Peptides Epitope Mapping |
| Zdroj: | The Journal of Immunology. 161:448-457 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Japanese cedar pollinosis is caused by exposure to Japanese cedar (Cryptomeria japonica) pollen, of which two components, Cry j 1 and Cry j 2, are believed to be the major allergens. T cell lines specific to either Cry j 1 or rCry j 2 were reactive to various portions of each panel of overlapping peptides derived from Cry j 1 or Cry j 2. Two peptides, p211–225 and p108–120, from among six major T cell epitopes identified in Cry j 1 sequence, and three peptides, p182–200, p344–355, and p66–80, from among five in Cry j 2, were chosen to design an artificial polypeptide (named Cry-consensus) based on a difference among the types of the restriction molecules capable of presenting these peptides. After construction of a DNA encoding these peptides in order, Cry-consensus was expressed in Escherichia coli. Five of six T cell epitopes, except for Cry j 2 p344–355, in Cry-consensus were recognized by the T cell clones specific to each peptide. PBMC from allergic patients induced higher proliferation under stimulation from Cry-consensus than individual peptides. Eighty-eight percent of the PBMC (15 of 17) showed proliferation under the Cry-consensus stimulation. Thus, several major T cell epitopes from Cry j 1 and Cry j 2 can be chosen in the design of peptide-based immunotherapeutics for the management of Japanese cedar pollinosis in subjects having various types of HLA class II molecules. |
| Databáze: | OpenAIRE |
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