The effect of PrP Sc accumulation on inflammatory gene expression within sheep peripheral lymphoid tissue
| Autor: | Anton G. Gossner, John Hopkins |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
PrPSc Proteins
animal diseases Scrapie Biology Microbiology Article Gene expression Sheep scrapie lymph node inflammation transcriptome medicine Animals Lymph node Regulation of gene expression Inflammation General Veterinary integumentary system Microarray analysis techniques Gene Expression Profiling Brain hemic and immune systems General Medicine respiratory system Microarray Analysis veterinary(all) Gene expression profiling Lymphatic system medicine.anatomical_structure Gene Expression Regulation Immunology Disease Progression Lymph Lymph Nodes Transcriptome tissues New Zealand |
| Zdroj: | Veterinary Microbiology Gossner, A G & Hopkins, J 2015, ' The effect of PrP(Sc) accumulation on inflammatory gene expression within sheep peripheral lymphoid tissue ', Veterinary Microbiology, vol. 181, no. 3-4, pp. 204-211 . https://doi.org/10.1016/j.vetmic.2015.10.013 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2015.10.013 |
| Popis: | Highlights • Arrays quantified gene expression in peripheral LNs during sheep scrapie. • Disease progression associated with alterations of inflammatory gene expression. • Lymph node response contrasts with response of CNS. • Step changes to gene expression after the detection of PrPSc in peripheral LNs. Accumulation of the misfolded prion protein, PrPSc in the central nervous system (CNS) is strongly linked to progressive neurodegenerative disease. For many transmissible spongiform encephalopathies (TSEs), peripheral lymphoid tissue is an important site of PrPSc amplification but without gross immunological consequence. Susceptible VRQ homozygous New Zealand Cheviot sheep were infected with SSBP/1 scrapie by inoculation in the drainage area of the prescapular lymph nodes. The earliest time that PrPSc was consistently detected by immunohistology in these nodes was D50 post infection. This transcriptomic study of lymph node taken before (D10) and after (D50) the detection of PrPSc, aimed to identify the genes and physiological pathways affected by disease progression within the nodes as assessed by PrPSc detection. Affymetrix Ovine Gene arrays identified 75 and 80 genes as differentially-expressed at D10 and D50, respectively, in comparison with control sheep inoculated with uninfected brain homogenate. Approximately 70% of these were repressed at each time point. RT-qPCR analysis of seven genes showed statistically significant correlation with the array data, although the results for IL1RN and TGIF were different between the two technologies. The ingenuity pathway analysis (IPA) and general low level of repression of gene expression in lymphoid tissue, including many inflammatory genes, contrasts with the pro-inflammatory and pro-apoptotic events that occur within the CNS at equivalent stages of disease progression as assessed by PrPSc accumulation. |
| Databáze: | OpenAIRE |
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