Arabidopsis mutants impaired in glutathione biosynthesis exhibit higher sensitivity towards the glucosinolate hydrolysis product allyl-isothiocyanate
| Autor: | János Urbancsok, Ralph Kissen, Atle M. Bones |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Glucosinolates Mutant Arabidopsis lcsh:Medicine Plant Roots 01 natural sciences Article Fluorescence 03 medical and health sciences chemistry.chemical_compound Isothiocyanates Arabidopsis thaliana Biomass lcsh:Science Buthionine Sulfoximine Multidisciplinary biology Arabidopsis Proteins Hydrolysis lcsh:R food and beverages Biological Transport Glutathione Plants Genetically Modified biology.organism_classification Allyl isothiocyanate 030104 developmental biology chemistry Biochemistry Seedlings Sulfoxides Glucosinolate Mutation lcsh:Q Growth inhibition Oxidation-Reduction 010606 plant biology & botany Sulforaphane |
| Zdroj: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-13 (2018) |
| Popis: | Upon tissue damage the plant secondary metabolites glucosinolates can generate various hydrolysis products, including isothiocyanates (ITCs). Their role in plant defence against insects and pest and their potential health benefits have been well documented, but our knowledge regarding the endogenous molecular mechanisms of their effect in plants is limited. Here we investigated the effect of allyl-isothiocyanate (AITC) on Arabidopsis thaliana mutants impaired in homeostasis of the low-molecular weight thiol glutathione. We show that glutathione is important for the AITC-induced physiological responses, since mutants deficient in glutathione biosynthesis displayed a lower biomass and higher root growth inhibition than WT seedlings. These mutants were also more susceptible than WT to another ITC, sulforaphane. Sulforaphane was however more potent in inhibiting root growth than AITC. Combining AITC with the glutathione biosynthesis inhibitor L-buthionine-sulfoximine (BSO) led to an even stronger phenotype than observed for the single treatments. Furthermore, transgenic plants expressing the redox-sensitive fluorescent biomarker roGFP2 indicated more oxidative conditions during AITC treatment. Taken together, we provide genetic evidence that glutathione plays an important role in AITC-induced growth inhibition, although further studies need to be conducted to reveal the underlying mechanisms. © The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License. |
| Databáze: | OpenAIRE |
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