Improved detection of gene-microbe interactions in the mouse skin microbiota using high-resolution QTL mapping of 16S rRNA transcripts
| Autor: | Meriem Belheouane, Yask Gupta, Saleh M. Ibrahim, John F. Baines, Sven Künzel |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
QTL mapping
0301 basic medicine Microbiology (medical) Host–pathogen interaction Quantitative Trait Loci Single-nucleotide polymorphism Skin microbiota Quantitative trait locus Biology Polymorphism Single Nucleotide Microbiology lcsh:Microbial ecology DNA sequencing Mice 03 medical and health sciences RNA Ribosomal 16S Genetic variation medicine Animals Skin cancer Gene Phylogeny Skin Genetics Bacteria Sequence Analysis RNA Research Sequence Analysis DNA Ribosomal RNA medicine.disease 030104 developmental biology Host-Pathogen Interactions lcsh:QR100-130 16S rRNA transcript |
| Zdroj: | Microbiome Microbiome, Vol 5, Iss 1, Pp 1-17 (2017) |
| Popis: | Background Recent studies highlight the utility of quantitative trait locus (QTL) mapping for determining the contribution of host genetics to interindividual variation in the microbiota. We previously demonstrated that similar to the gut microbiota, abundances of bacterial taxa in the skin are significantly influenced by host genetic variation. In this study, we analyzed the skin microbiota of mice from the 15th generation of an advanced intercross line using a novel approach of extending bacterial trait mapping to both the 16S rRNA gene copy (DNA) and transcript (RNA) levels, which reflect relative bacterial cell number and activity, respectively. Results Remarkably, the combination of highly recombined individuals and 53,203 informative SNPs allowed the identification of genomic intervals as small as |
| Databáze: | OpenAIRE |
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