Fibroblast growth factor 12 is expressed in spiral and vestibular ganglia and necessary for auditory and equilibrium function

Autor:	Yoshihisa Koyama, Manabu Taniguchi, Yusuke Ishida, Takao Imai, Makoto Kondo, Shoichi Shimada, Takashi Sato, Tetsuo Morihana, Kazuya Ohata, Yukiko Hanada, Yasumitsu Takimoto, Hidenori Inohara, Yukiko Nakamura, Yoshiyuki Ozono
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	0301 basic medicine Scarpa's ganglion lcsh:Medicine Biology Vestibular Nerve Fibroblast growth factor Article 03 medical and health sciences Mice Hearing Hair Cells Auditory medicine Evoked Potentials Auditory Brain Stem otorhinolaryngologic diseases Animals Inner ear RNA Messenger lcsh:Science Spiral ganglion Vestibular system Mice Knockout Neurons Multidisciplinary Absolute threshold of hearing cDNA library lcsh:R Cell biology Fibroblast Growth Factors Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Auditory brainstem response Ear Inner lcsh:Q sense organs CRISPR-Cas Systems Spiral Ganglion
Zdroj:	Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018) Scientific Reports
ISSN:	2045-2322
Popis:	We investigated fibroblast growth factor 12 (FGF12) as a transcript enriched in the inner ear by searching published cDNA library databases. FGF12 is a fibroblast growth factor homologous factor, a subset of the FGF superfamily. To date, its localisation and function in the inner ear have not been determined. Here, we show that FGF12 mRNA is localised in spiral ganglion neurons (SGNs) and the vestibular ganglion. We also show that FGF12 protein is localised in SGNs, the vestibular ganglion, and nerve fibres extending beneath hair cells. Moreover, we investigated FGF12 function in auditory and vestibular systems using Fgf12-knockout (FGF12-KO) mice generated with CRISPR/Cas9 technology. Our results show that the inner ear morphology of FGF12-KO mice is not significantly different compared with wild-type mice. However, FGF12-KO mice exhibited an increased hearing threshold, as measured by the auditory brainstem response, as well as deficits in rotarod and balance beam performance tests. These results suggest that FGF12 is necessary for normal auditory and equilibrium function.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76fe8fbbfbb443e935d8edbe85f92490 http://link.springer.com/article/10.1038/s41598-018-28618-0 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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