The study of acute leukemia cells by means of acridine orange staining and flow cytometry
| Autor: | Azra Raza, B. Lampkin, Harvey D. Preisler, Venu Gopal, J. Bokhari, Shripad Banavali |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Adult
Cancer Research Population Biology Monocytes Flow cytometry chemistry.chemical_compound Bone Marrow Reference Values medicine Humans Lymphocytes RNA Neoplasm education Child Acute leukemia education.field_of_study medicine.diagnostic_test Acridine orange Cell Cycle Granulocyte-Macrophage Colony-Stimulating Factor Hematology Precursor Cell Lymphoblastic Leukemia-Lymphoma medicine.disease Flow Cytometry Molecular biology Acridine Orange Recombinant Proteins Staining Leukemia Leukemia Myeloid Acute Granulocyte macrophage colony-stimulating factor medicine.anatomical_structure Oncology chemistry RNA Bone marrow medicine.drug Granulocytes |
| Zdroj: | Leukemialymphoma. 13(1-2) |
| ISSN: | 1042-8194 |
| Popis: | The studies described here explored the staining of acute leukemia cells with acridine orange (AO). The red fluorescence curve of AML specimens was usually bimodal, suggesting the presence of subpopulations of cells which have different RNA contents. In almost every AML specimen, small leukemic blast cells comprised at least part of the “low RNA content” sub-population. Residual granulocytes and lymphocytes also contributed to this population. Frequently, the green fluorescence, indicative of the binding of A0 to DNA, was slightly less in these cells than in the majority of cells present. There was no evidence however, that the leukemia cells with these characteristics represented a G0 or kinetically quiescent population of cells. In ALL specimens, the presence of multiple cytogenetically distinct clones was easily detectable in AO stained specimens. The red fluorescence curve of G0/G1 ALL cells was unimodal. |
| Databáze: | OpenAIRE |
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