In-vitro and in-vivo monitoring of gold(III) ions from intermediate metabolite of sodium aurothiomalate through water-soluble ruthenium (II) complex-based luminescent probe
| Autor: | Lixia Wang, Bo Zhang, Chenglin Hong, Shichao Han, Shaowei Sun, Jia Wen, Shiguo Sun, Zhenzhen Xie, Xiling Deng, Jing Zhang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Lipopolysaccharides
Biocompatibility Cell Survival Metabolite chemistry.chemical_element 01 natural sciences Biochemistry Gold Sodium Thiomalate Mice chemistry.chemical_compound In vivo Drug Discovery medicine Animals Humans Molecular Biology Zebrafish Fluorescent Dyes chemistry.chemical_classification Aqueous solution Molecular Structure 010405 organic chemistry Organic Chemistry Hep G2 Cells Combinatorial chemistry Sodium aurothiomalate 0104 chemical sciences Ruthenium 010404 medicinal & biomolecular chemistry RAW 264.7 Cells chemistry Thiol Ruthenium Compounds Gold Single-Cell Analysis Drug metabolism medicine.drug |
| Zdroj: | Bioorganic Chemistry. 110:104749 |
| ISSN: | 0045-2068 |
| DOI: | 10.1016/j.bioorg.2021.104749 |
| Popis: | Real-time monitoring of drug metabolism in vivo is of great significance to drug development and toxicology research. The purpose of this study is to establish a rapid and visual in vivo detection method for the detection of an intermediate metabolite of the gold (I) drug. Gold (I) drugs such as sodium aurothiomalate (AuTM) have anti-inflammatory effects in the treatment of rheumatoid arthritis. Gold(III) ions (Au3+) are the intermediate metabolite of gold medicine, and they are also the leading factor of side effects in the treatment of patients. However, the rapid reduction of Au3+ to Au+ by thiol proteins in organisms limits the in-depth study of metabolism of gold drugs in vivo. Here we describe a luminescence Au3+ probe (RA) based on ruthenium (II) complex for detecting Au3+ in vitro and in vivo. RA with large Stokes shift, good water solubility and biocompatibility was successfully applied to detect Au3+ in living cells and vivo by luminescence imaging, and to trap the fluctuation of Au3+ level produced by gold (I) medicine. More importantly, the luminescent probe was used to the detection of the intermediate metabolites of gold (I) drugs for the first time. Overall, this work offers a new detection tool/method for a deeper study of gold (I) drugs metabolite. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect