Improvement of saccharide yield from wood by simultaneous enzymatic delignification and saccharification using a ligninolytic enzyme and cellulase
| Autor: | Hirokazu Kawagishi, Toshio Mori, Hirofumi Hirai, Kohei Ikeda |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Bioengineering Cellulase Phanerochaete Saccharification complex mixtures 01 natural sciences Applied Microbiology and Biotechnology Lignin Phanerochaete sordida YK-624 03 medical and health sciences Hydrolysis chemistry.chemical_compound Manganese peroxidase 010608 biotechnology chemistry.chemical_classification Reaction conditions biology technology industry and agriculture In vitro degradation Biorefinery Pulp and paper industry Wood 030104 developmental biology Enzyme Co-oxidants chemistry Yield (chemistry) biology.protein Biotechnology |
| Zdroj: | Journal of Bioscience and Bioengineering. 132(3):213-219 |
| ISSN: | 1389-1723 |
| Popis: | White-rot fungi are thought to hold promise for development of a delignification pretreatment process for wood biorefinery that is less energy-consuming than current processes. However, the reaction must take place over weeks and consumes non-neglectable amounts of saccharides. To establish a biological process for wood biorefinery would first require establishment of an enzymatic approach to delignification. Such an approach has the potential to lower costs and reduce saccharide loss. Here, we attempted enzymatic delignification reactions using manganese peroxidases (MnP), a lignin-degrading enzyme, under several reaction conditions. The delignification rate from beech wood meal (particle size45 μm) of up to 11.0% in 48 h was reached in a MnP reaction supplemented with multiple co-oxidants, glucose, glucose oxidase (GOD) and commercial cellulase. An additional 48-h reaction using fresh MnP/co-oxidants increased the delignification rate to 14.2%. Simultaneous enzymatic delignification and saccharification, which occurs without a need for glucose supplementation, successfully improved the glucose yield to 160% of the reaction without MnP. Development of a more accurate imitation of the mechanisms of delignification that occurs in white-rot fungi has the potential to improve the monosaccharide yield resulting from simultaneous delignification and saccharification. |
| Databáze: | OpenAIRE |
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