Myeloperoxidase is required for neutrophil extracellular trap formation: implications for innate immunity
| Autor: | Venizelos Papayannopoulos, Joachim Roesler, Dominique Reumaux, William M. Nauseef, Volker Wahn, Kathleen D. Metzler, Tobias A. Fuchs, Arturo Zychlinsky, Ilka Schulze |
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| Rok vydání: | 2011 |
| Předmět: |
Extracellular Traps
Genotype Neutrophils animal diseases Blotting Western Immunology Blood Donors Granulocyte Biochemistry Microbiology Phagocytes Granulocytes and Myelopoiesis Immunity Candida albicans medicine Extracellular Humans Cells Cultured Peroxidase Aniline Compounds Innate immune system biology Cell Biology Hematology Neutrophil extracellular traps biology.organism_classification Immunity Innate medicine.anatomical_structure Microscopy Fluorescence Myeloperoxidase Host-Pathogen Interactions Mutation biology.protein Tetradecanoylphorbol Acetate Extracellular Space |
| Zdroj: | Blood. 117:953-959 |
| ISSN: | 1528-0020 0006-4971 |
| Popis: | The granule enzyme myeloperoxidase (MPO) plays an important role in neutrophil antimicrobial responses. However, the severity of immunodeficiency in patients carrying mutations in MPO is variable. Serious microbial infections, especially with Candida species, have been observed in a subset of completely MPO-deficient patients. Here we show that neutrophils from donors who are completely deficient in MPO fail to form neutrophil extracellular traps (NETs), indicating that MPO is required for NET formation. In contrast, neutrophils from partially MPO-deficient donors make NETs, and pharmacological inhibition of MPO only delays and reduces NET formation. Extracellular products of MPO do not rescue NET formation, suggesting that MPO acts cell-autonomously. Finally, NET-dependent inhibition of Candida albicans growth is compromised in MPO-deficient neutrophils. The inability to form NETs may contribute in part to the host defense defects observed in completely MPO-deficient individuals. |
| Databáze: | OpenAIRE |
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