Measuring behavioral and endocrine responses to novelty stress in adult zebrafish
| Autor: | Keith Wong, Brett Bartels, Peter C. Hart, Christopher Suciu, Thomas Gilder, Salem Elkhayat, Sudipta Roy, John DiLeo, Jason Goodspeed, Kyung Min Chung, Julia Tan, Eli Utterback, Marco Elegante, Jonathan Cachat, Kevin Frank, Ashley Denmark, Leah Grossman, David Tien, Siddharth Gaikwad, Ferdous Kadri, Adam Stewart, Katie Chang, Evan J. Kyzar, Nadine Wu, Allan V. Kalueff |
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| Rok vydání: | 2010 |
| Předmět: |
biology
Behavior Animal Hydrocortisone ved/biology fungi ved/biology.organism_classification_rank.species Danio Novelty Neurosciences biology.organism_classification General Biochemistry Genetics and Molecular Biology Freezing behavior Stress Physiological Models Animal medicine Endocrine system Anxiety Animals medicine.symptom Model organism Zebrafish Neuroscience Cortisol level |
| Zdroj: | Nature protocols. 5(11) |
| ISSN: | 1750-2799 |
| Popis: | Several behavioral assays are currently used for high-throughput neurophenotyping and screening of genetic mutations and psychotropic drugs in zebrafish (Danio rerio). In this protocol, we describe a battery of two assays to characterize anxiety-related behavioral and endocrine phenotypes in adult zebrafish. Here, we detail how to use the 'novel tank' test to assess behavioral indices of anxiety (including reduced exploration, increased freezing behavior and erratic movement), which are quantifiable using manual registration and computer-aided video-tracking analyses. In addition, we describe how to analyze whole-body zebrafish cortisol concentrations that correspond to their behavior in the novel tank test. This protocol is an easy, inexpensive and effective alternative to other methods of measuring stress responses in zebrafish, thus enabling the rapid acquisition and analysis of large amounts of data. As will be shown here, fish anxiety-like behavior can be either attenuated or exaggerated depending on stress or drug exposure, with cortisol levels generally expected to parallel anxiety behaviors. This protocol can be completed over the course of 2 d, with a variable testing duration depending on the number of fish used. |
| Databáze: | OpenAIRE |
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