Leukotrienes and isocyanate-induced asthma: a pilot study
| Autor: | S. Pelissier, Caroline Tremblay, Catherine Lemière, Jana Stankova, Maryse Thivierge, Simone Chaboillez, Marek Rola-Pleszczynski |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Adult
Male Leukotrienes Allergy Neutrophils Leukotriene B4 Receptor expression Immunology Receptors Leukotriene B4 Pilot Projects Bronchial Provocation Tests chemistry.chemical_compound medicine Humans Immunology and Allergy Cysteine Asthma Leukotriene Receptors Purinergic P2 business.industry Macrophages Interleukin-8 Leukotriene B4 receptor Sputum Middle Aged respiratory system medicine.disease respiratory tract diseases Occupational Diseases chemistry Female lipids (amino acids peptides and proteins) medicine.symptom business Occupational asthma Isocyanates |
| Zdroj: | Clinical Experimental Allergy. 34:1684-1689 |
| ISSN: | 1365-2222 0954-7894 |
| DOI: | 10.1111/j.1365-2222.2004.02102.x |
| Popis: | Summary Background The role of leukotrienes (LTs) in the pathophysiology of isocyanate-induced asthma is not well known. Objective We sought to characterize the type of airway inflammation induced by exposure to isocyanates and to investigate whether exposure to isocyanates induced an increase in LT receptor cysteinyl leukotriene ((CysLT)1, CysLT2 and leukotriene B4 receptor (BLT1)) expression, as well as a release of LT (LTC4 and leukotriene B4 (LTB4)) and IL-8 in both asthmatics with isocyanate-induced asthma and healthy subjects. Methods We investigated eight subjects with isocyanate-induced asthma and eight healthy subjects. Both groups underwent specific inhalation challenges to isocyanates in the laboratory. Induced sputum was collected before and after exposure to isocyanates. CysLT1, CysLT2 and BLT1 expression was assessed by flow cytometry, whereas LTC4, LTB4 and IL-8 were measured in the sputum supernatants by enzyme immunoassay. Results Exposure to isocyanates induced an increase in sputum neutrophils only in subjects with occupational asthma. There was a significant increase in CysLT1 and BLT1 receptor expression, as well as a release of LTB4 and IL-8 after exposure to isocyanates compared with the baseline, only in subjects with isocyanate-induced asthma, whereas there was no increase in LTC4. Exposure to isocyanates did not induce any change in LT receptor expression nor in the levels of LTC4, LTB4 and IL-8, in healthy subjects. Conclusion The neutrophilia observed after exposure to isocyanates is likely to be related to the release of LTB4, probably enhanced by the increased expression of BLT1 on neutrophils as well as by the release of IL-8. The significance of the increase of CysLT1 receptor expression on neutrophils is unknown and needs further investigation. |
| Databáze: | OpenAIRE |
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