High Purity Isolation of Low Density Neutrophils Casts Doubt on Their Exceptionality in Health and Disease
| Autor: | Frances Llanwarne, Robert D. Gray, Gareth Hardisty, Emily Gwyer Findlay, Danielle Minns, Donald J. Davidson, Jonathan L Gillan |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cystic Fibrosis Neutrophils T-Lymphocytes Apoptosis Cell Separation Lymphocyte Activation Extracellular Traps Neutrophil Activation Receptors Interleukin-8B cystic fibrosis Leukocyte Count 0302 clinical medicine neutrophils Immunopathology Immunology and Allergy Homeostasis Cells Cultured Original Research low-density neutrophils Flow Cytometry Healthy Volunteers medicine.anatomical_structure Phenotype Cellular Microenvironment Tumor necrosis factor alpha medicine.symptom T cell Immunology neutrophil extracellular traps Inflammation CD16 03 medical and health sciences Interferon-gamma Antigens CD medicine Centrifugation Density Gradient Humans Cell Proliferation business.industry Neutrophil extracellular traps RC581-607 Neutrophilia 030104 developmental biology neutrophil T cell interactions inflammation Case-Control Studies Immunologic diseases. Allergy business Reactive Oxygen Species CD8 Leukocyte Disorders 030215 immunology |
| Zdroj: | Frontiers in Immunology Hardisty, G, Llanwarne, F, Minns, D, Gillan, J, Davidson, D J, Gwyer Findlay, E L & Gray, R D 2021, ' High purity isolation of low density neutrophils casts doubt on their exceptionality in health and disease ', Frontiers in Immunology, vol. 12, 625922 . https://doi.org/10.3389/fimmu.2021.625922 Frontiers in Immunology, Vol 12 (2021) |
| ISSN: | 1664-3224 |
| DOI: | 10.3389/fimmu.2021.625922 |
| Popis: | Low density neutrophils (LDNs) are described in a number of inflammatory conditions, cancers and infections and associated with immunopathology, and a mechanistic role in disease. The role of LDNs at homeostasis in healthy individuals has not been investigated. We have developed an isolation protocol that generates high purity LDNs from healthy donors. Healthy LDNs were identical to healthy normal density neutrophils (NDNs), aside from reduced neutrophil extracellular trap formation. CD66b, CD16, CD15, CD10, CD54, CD62L, CXCR2, CD47 and CD11b were expressed at equivalent levels in healthy LDNs and NDNs and underwent apoptosis and ROS production interchangeably. Healthy LDNs had no differential effect on CD4+ or CD8+ T cell proliferation or IFNγ production compared with NDNs. LDNs were generated from healthy NDNs in vitro by activation with TNF, LPS or fMLF, suggesting a mechanism of LDN generation in disease however, we show neutrophilia in people with Cystic Fibrosis (CF) was not due to increased LDNs. LDNs are present in the neutrophil pool at homeostasis and have limited functional differences to NDNs. We conclude that increased LDN numbers in disease reflect the specific pathology or inflammatory environment and that neutrophil density alone is inadequate to classify discrete functional populations of neutrophils. |
| Databáze: | OpenAIRE |
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