Some biochemical properties of the components of Staphylococcus aureus binding to human platelets
| Autor: | Yoshitoshi Ichiman, Yukio Ohshima, Toshichika Ohtomo, Yukio Usui, Jingoro Shimada |
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| Rok vydání: | 1997 |
| Předmět: |
Blood Platelets
Staphylococcus aureus Micrococcaceae Hot Temperature Sonication Immunology Carbohydrates Biotin Succinimides Pronase medicine.disease_cause Cell Fractionation Bacterial Adhesion Endopeptidases medicine Humans Platelet Staphylococcal Protein A biology Cell Membrane biology.organism_classification Trypsin Proteinase K Biochemistry biology.protein Bacteria medicine.drug |
| Zdroj: | Zentralblatt fur Bakteriologie : international journal of medical microbiology. 286(1) |
| ISSN: | 0934-8840 |
| Popis: | The binding properties of Staphylococcus aureus in relation to human platelets were investigated. Protease digestion (pronase E, proteinase K, trypsin), heat treatment (80 degrees C, 30 min), and sonication for 5 min significantly reduced the binding abilities of the staphylococcal cells to 0% (p.01), 50 +/- 5% (p.05), and 38 +/- 9% (p.05), respectively, while mixed glycosidases did not. Inhibition experiments indicated that protein A and various sugars were ineffective. A binding study using biotinylated cell surface fractions extracted from the whole cells of S. aureus indicated that the proteins having apparent molecular weights of 14400 and 16500 estimated by sodium dodecylsulfate-polyacrylamide gel electrophoresis were involved in the binding between S. aureus and human platelets. |
| Databáze: | OpenAIRE |
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