The effects of pneumoperitoneum and controlled ventilation on peritoneal lymphatic bacterial clearance: experimental results in rats

Autor: Lycia M. J Mimica, Belchor Fontes, Armando Angelo Casaroli, Samir Rasslan
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Zdroj: Clinics, Volume: 66, Issue: 9, Pages: 1621-1625, Published: 2011
Clinics, Vol 66, Iss 9, Pp 1621-1625 (2011)
Clinics; v. 66 n. 9 (2011); 1621-1625
Clinics; Vol. 66 Núm. 9 (2011); 1621-1625
Clinics; Vol. 66 No. 9 (2011); 1621-1625
Clinics
Universidade de São Paulo (USP)
instacron:USP
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
ISSN: 1980-5322
1807-5932
Popis: OBJECTIVE: To evaluate the effect of pneumoperitoneum, both alone and in combination with controlled ventilation, on peritoneal lymphatic bacterial clearance using a rat bacterial peritonitis model. METHOD: A total of 69 male Wistar rats were intraperitoneally inoculated with an Escherichia coli solution (109 colony-forming units (cfu)/mL) and divided into three groups of 23 animals each: A (control group), B (pneumoperitoneum under 5 mmHg of constant pressure), and C (endotracheal intubation, controlled ventilation, and pneumoperitoneum as in Group B). The animals were sacrificed after 30 min under these conditions, and blood, mediastinal ganglia, lungs, peritoneum, liver, and spleen cultures were performed. RESULTS: Statistical analyses comparing the number of cfu/sample in each of the cultures showed that no differences existed between the three groups. CONCLUSION: Based on our results, we concluded that pneumoperitoneum, either alone or in association with mechanical ventilation, did not modify the bacterial clearance through the diaphragmatic lymphatic system of the peritoneal cavity.
Databáze: OpenAIRE