Comparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattle
| Autor: | Aideen Kennedy, Grace Mulcahy, Mary Sekiya, Rosemary Rea, Ana Maria Martinez-Ibeas, N. Byrne, Riona Sayers, Maria Pia Munita |
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| Přispěvatelé: | Department of Agriculture, Food and the Marine, Dairy Research Ireland, 13/ S/405 |
| Rok vydání: | 2019 |
| Předmět: |
Fascioliasis
Veterinary medicine 040301 veterinary sciences 030231 tropical medicine Population Cattle Diseases Enzyme-Linked Immunosorbent Assay Comparison Beef cattle Flukicides Sensitivity and Specificity Serology 0403 veterinary science 03 medical and health sciences Sensitivity 0302 clinical medicine Hepatica parasitic diseases medicine Animals Bulk tank Fasciola hepatica education education.field_of_study lcsh:Veterinary medicine General Veterinary biology seasonality 04 agricultural and veterinary sciences General Medicine biology.organism_classification Treatment Triclabendazole Specificity Herd lcsh:SF600-1100 Cattle ELISA Ireland Research Article medicine.drug |
| Zdroj: | BMC Veterinary Research, Vol 15, Iss 1, Pp 1-12 (2019) BMC Veterinary Research |
| ISSN: | 1746-6148 |
| Popis: | Background Fasciola hepatica is a liver parasite of mammals and it results in poor welfare outcomes and economic losses in ruminants. While faecal egg count is the test most commonly used for diagnosis, it does not indicate presence of migrating immature stages. Serological techniques increase sensitivity at all stages of the liver fluke infection. The aim of this study was to compare four commercially available ELISA tests for the diagnosis of F. hepatica. For this purpose, we tested three sample types; (i) known F. hepatica status sera from an experimental infection for the comparison of sensitivities and specificities, (ii) sera from pre- and post-flukicide-treated (albendazole, closantel, nitroxynil and triclabendazole) beef cattle to contrast the differences of seropositivity before and after treatment, and (iii) bulk tank milk samples from dairy herds sampled during high and low F. hepatica exposure periods for assessing seasonal variations with the four tests available. Samples were tested using ELISA kits supplied by four manufacturers (Ildana Biotech, IDEXX, Svanova, and Bio-X). Samples were analysed simultaneously and in duplicate. Results In the control population Ildana, IDEXX and Bio-X presented 100% sensitivity (Se) and specificity (Sp), Svanovir presented a Se of 59% and a Sp of 96%. In flukicide-treated beef cattle, kits highlighted decreasing antibody levels 90 days post-treatment in variable degrees. Finally, bulk milk showed a significant decrease in ELISA value between high and low fluke exposure periods with all tests studied. Conclusions Se and Sp found in the present study, confirm that Ildana, IDEXX and Bio-X are accurate for the detection of F. hepatica exposure in Irish cattle. Svanovir Se and Sp in this population, indicate that a larger study is necessary to confirm this test characteristic in Irish herds. In post-treatment use, Bio-X showed a consistent and significant decrease of ELISA value in all groups treated, denoting to be a reliable tool for assessing treatment effect at 90 days post-treatment. Finally, all tests showed to be a reliable tool for the F. hepatica monitoring of high and low exposure seasons, using bulk tank milk samples. |
| Databáze: | OpenAIRE |
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