Diethylstilbestrol stimulates ornithine decarboxylase in kidney cells in culture
| Autor: | Young C. Lin, Janet M. Loring, Claude A. Villee |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Adenosylmethionine Decarboxylase
medicine.medical_specialty Carboxy-Lyases Biophysics Diethylstilbestrol Hamster Cycloheximide Biology Kidney Ornithine Decarboxylase Biochemistry Ornithine decarboxylase chemistry.chemical_compound Cricetinae Internal medicine medicine Protein biosynthesis Animals Molecular Biology Cells Cultured Ornithine decarboxylase antizyme Muscles Methionine decarboxylase Cell Biology Molecular biology Endocrinology chemistry Steroids medicine.drug Hormone |
| Zdroj: | Biochemical and Biophysical Research Communications. 95:1393-1403 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/s0006-291x(80)80052-0 |
| Popis: | Summary Diethylstilbestrol, estradiol-17β, progesterone or testosterone at concentrations of 10 −9 M, but not a number of other hormones tested, increase the activity of ornithine decarboxylase in hamster kidney cells maintained in culture with the peak in activity occurring three to six hours after addition of hormones. S-adenosyl methionine decarboxylase of these cells is also stimulated by DES. The DES induced increase in the activity of ornithine decarboxylase and of S-adenosyl methionine decarboxylase involves protein synthesis — it is inhibited by cycloheximide — but does not involve RNA synthesis at that moment — it is not inhibited by actinomycin D. This suggests that the mRNA for ODC and SAMDC may be synthesized earlier and retained in the cell in some inactive form until released by the estrogenic stimulus. |
| Databáze: | OpenAIRE |
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