Improved ATP methodology for biomass assays

Autor:	Anne Weyens van Witzenburg, Eric Schram
Rok vydání:	1989
Předmět:	Bacteria Ecology Apyrase Hydrolysis ATPase Colony Count Microbial Temperature Biophysics Biomass Biology Kinetics Adenosine Triphosphate Biochemistry Chemistry (miscellaneous) Luminescent Measurements biology.protein Luciferase Luciferases Inhibitory effect Constant light
Zdroj:	Journal of Bioluminescence and Chemiluminescence. 4:390-398
ISSN:	1099-1271 0884-3996
DOI:	10.1002/bio.1170040153
Popis:	ATP methodology needs to be further standardized and improved in order to avoid the pitfalls that have sometimes hampered its application to biomass assays. The following steps have been reconsidered as far as the bacteriological applications is concerned: (a) destruction of free and somatic ATP: replacement of apyrase by mammalian ATPase, more readily accessible to specific inhibition; (b) extraction of bacterial ATP: protection of luciferase by lipids against inhibitory effect of cationic detergents with production of a constant light response. New methods are proposed for the calibration of luminometers and for the matching of sample holders in multichannel instruments. The limit of sensitivity of ATP assays is discussed in the light of currently available reagents and instruments.
Databáze:	OpenAIRE
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