Serum starvation and growth factor receptor expression in vascular smooth muscle cells
| Autor: | Andreas Hannekum, Michael Torzewski, Jan Torzewski, Nikolaus Marx, Vinzenz Hombach, Peter Guilliard, Andrea Bucher, Thomas P. Zwaka, Oliver Zimmermann |
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| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
Vascular smooth muscle Physiology Cell Becaplermin Down-Regulation Coronary Artery Disease Biology Culture Media Serum-Free Muscle Smooth Vascular Receptor IGF Type 1 Receptor Platelet-Derived Growth Factor beta Downregulation and upregulation Growth factor receptor Internal medicine medicine Humans Receptor Cells Cultured Cell Proliferation Platelet-Derived Growth Factor Cell growth Vascular disease Proto-Oncogene Proteins c-sis medicine.disease Up-Regulation medicine.anatomical_structure Endocrinology Cardiology and Cardiovascular Medicine |
| Zdroj: | Journal of vascular research. 43(2) |
| ISSN: | 1018-1172 |
| Popis: | Background: Smooth muscle cell (SMC) proliferation in atherosclerosis is regulated through the interaction of growth factors like platelet-derived growth factor-BB (PDGF-BB) and insulin-like growth factor-1 (IGF-1) and their receptors (R). We hypothesized that serum starvation of SMCs may affect PDGFβ-R and IGF-1-R expression and, consequently, the effect of their cognate ligands on SMC survival/proliferation. Methods and Results: Serum starvation significantly increases PDGFβ-R but not IGF-1-R mRNA and protein expression in SMCs. PDGF-BB stimulates cell survival but not proliferation in serum-starved SMCs of the synthetic phenotype, whereas SMCs of the contractile phenotype respond to PDGF-BB by a significant increase in proliferation. Immunohistochemical analysis of coronary atherosclerotic lesions reveals PDGFβ-R expression in SMCs in the lamina fibromuscularis, but not in the media and in healthy parts of the arterial wall. No such differential expression was observed for IGF-1-R. Conclusions: Differential regulation of PDGFβ-R and IGF-1-R expression by serum starvation might represent a mechanism for the control of SMC survival/proliferation in atherogenesis and restenosis. The distribution of PDGFβ-Rs and IGF-1-Rs in atherosclerotic lesions may indicate an effect of serum starvation on SMCs in the arterial wall. |
| Databáze: | OpenAIRE |
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