High field gradient targeting of magnetic nanoparticle-loaded endothelial cells to the surfaces of steel stents
| Autor: | Michael Chorny, Robert J. Levy, Darryl N. Williams, Ivan S. Alferiev, Ben Yellen, Boris Polyak, Gary Friedman, Ilia Fishbein |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Materials science Polymers Carotid arteries medicine.medical_treatment Cell- and Tissue-Based Therapy Metal Nanoparticles Nanoparticle Biocompatible Materials Superparamagnetic nanoparticles Models Biological Rats Sprague-Dawley Magnetics Optical imaging In vivo medicine Animals Nanotechnology Aorta Multidisciplinary Endothelial Cells Stent Genetic Therapy Anatomy Biological Sciences equipment and supplies In vitro Rats Carotid Arteries Steel Cattle Stents High field human activities Biomedical engineering |
| Zdroj: | Proceedings of the National Academy of Sciences. 105:698-703 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.0708338105 |
| Popis: | A cell delivery strategy was investigated that was hypothesized to enable magnetic targeting of endothelial cells to the steel surfaces of intraarterial stents because of the following mechanisms: ( i ) preloading cells with biodegradable polymeric superparamagnetic nanoparticles (MNPs), thereby rendering the cells magnetically responsive; and ( ii ) the induction of both magnetic field gradients around the wires of a steel stent and magnetic moments within MNPs because of a uniform external magnetic field, thereby targeting MNP-laden cells to the stent wires. In vitro studies demonstrated that MNP-loaded bovine aortic endothelial cells (BAECs) could be magnetically targeted to steel stent wires. In vivo MNP-loaded BAECs transduced with adenoviruses expressing luciferase (Luc) were targeted to stents deployed in rat carotid arteries in the presence of a uniform magnetic field with significantly greater Luc expression, detected by in vivo optical imaging, than nonmagnetic controls. |
| Databáze: | OpenAIRE |
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