Autoimmune regulator act in synergism with thymocyte adhesion in the control of lncRNAs in medullary thymic epithelial cells
| Autor: | Romario S. Mascarenhas, Max Jordan Duarte, Geraldo Aleixo Silva Passos, Pedro Paranhos Tanaka, Cesar A. Speck-Hernandez, Amanda Freire Assis |
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| Rok vydání: | 2021 |
| Předmět: |
Time Factors
Transcription Genetic Immunology Thymus Gland Biology Cell Line Open Reading Frames Transcription (biology) microRNA Cell Adhesion Animals Cell adhesion Molecular Biology MALAT1 Thymocytes Gene Expression Profiling RNA Epithelial Cells Autoimmune regulator Cell biology Chromatin Mice Inbred C57BL Gene Expression Regulation RNA Long Noncoding XIST Transcription Factors |
| DOI: | 10.1101/2021.07.14.452339 |
| Popis: | The autoimmune regulator (Aire) gene in medullary thymic epithelial cells (mTECs) encodes the AIRE protein, which interacts with its partners within the nucleus. This “Aire complex” induces stalled RNA Pol II on chromatin to proceed with transcription elongation of a large set of messenger RNAs and microRNAs. Considering that RNA Pol II also transcribes long noncoding RNAs (lncRNAs), we hypothesized that Aire might be implicated in the upstream control of this RNA species. To test this, we employed a loss-of-function approach in which Aire knockout mTECs were compared to Aire wild-type mTECs for lncRNA transcriptional profiling both in vitro and in vivo model systems. RNA sequencing enables the differential expression profiling of lncRNAs when these cells adhere in vitro to thymocytes or do not adhere to them as a way to test the effect of cell adhesion. Sets of lncRNAs that are unique and that are shared in vitro and in vivo were identified. Among these, we found the Aire-dependent lncRNAs as for example, Platr28, Ifi30, Morrbid, Malat1, and Xist. This finding represents the first evidence that Aire mediates the transcription of lncRNAs in mTECs. Microarray hybridizations enabled us to observe that temporal thymocyte adhesion modulates the expression levels of such lncRNAs as Morrbid, Xist, and Fbxl12o after 36h of adhesion. This finding shows the existence of a synergistic mechanism involving a link between thymocyte adhesion, Aire, and lncRNAs in mTECs that might be important for immune self-representation. |
| Databáze: | OpenAIRE |
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