Heavy Metal Stress and Its Consequences on Exopolysaccharide (EPS)-Producing Pantoea agglomerans

Autor: Sunil H. Koli, Bhavana V. Mohite, Satish V. Patil
Rok vydání: 2018
Předmět:
0301 basic medicine
Microorganism
chemistry.chemical_element
Bioengineering
010501 environmental sciences
01 natural sciences
Applied Microbiology and Biotechnology
Biochemistry
Metal
03 medical and health sciences
Bioremediation
Bacterial Proteins
Microscopy
Electron
Transmission
Metals
Heavy
Spectroscopy
Fourier Transform Infrared
Molecular Biology
Phylogeny
Arsenic
0105 earth and related environmental sciences
Cadmium
biology
Pantoea
Chemistry
Polysaccharides
Bacterial
Biosorption
Spectrometry
X-Ray Emission
General Medicine
biology.organism_classification
Adaptation
Physiological
Copper
Pantoea agglomerans
Biodegradation
Environmental
030104 developmental biology
visual_art
Environmental chemistry
Microscopy
Electron
Scanning
visual_art.visual_art_medium
Carbohydrate Metabolism
Environmental Pollutants
Biotechnology
Zdroj: Applied Biochemistry and Biotechnology. 186:199-216
ISSN: 1559-0291
0273-2289
Popis: Currently, the heavy metal pollution is of grave concern, and the part of microorganism for metal bioremediation should take into account as an efficient and economic strategy. On this framework, the heavy metal stress consequences on exopolysaccharide (EPS)-producing agricultural isolate, Pantoea agglomerans, were studied. The EPS production is a protective response to stress to survive and grow in the metal-contaminated environment. P. agglomerans show tolerance and mucoid growth in the presence of heavy metals, i.e., mercury, copper, silver, arsenic, lead, chromium, and cadmium. EDX first confirmed the metal accumulation and further, FTIR determined the functional groups involved in metal binding. The ICP-AES identified the location of cell-bound and intracellular metal accumulation. Metal deposition on cell surface has released more Ca2+. The effect on bacterial morphology investigated with SEM and TEM revealed the sites of metal accumulation, as well as possible structural changes. Each heavy metal caused distinct change and accumulated on cell-bound EPS with some intracellular deposits. The metal stress caused a decrease in total protein content and increased in total carbohydrate with a boost in EPS. Thus, the performance of P. agglomerans under metal stress indicated a potential candidate for metal bioremediation. Graphical Abstract ᅟ.
Databáze: OpenAIRE
Externí odkaz: