Increased Expression of Interleukin‐1 Receptors on Fibroblasts Derived From Inflamed Gingiva
| Autor: | Takeshi Sueda, Chieko Kanda-Nakamura, Yuichi Izumi |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty medicine.drug_class Cell Gingiva Enzyme-Linked Immunosorbent Assay Monoclonal antibody Dinoprostone stomatognathic system medicine Humans Prostaglandin E2 Periodontitis Receptor Cells Cultured chemistry.chemical_classification Receptors Interleukin-1 Interleukin Fibroblasts Middle Aged Gingivitis stomatognathic diseases On cells Enzyme medicine.anatomical_structure chemistry Periodontics Female Positive staining Interleukin-1 medicine.drug |
| Zdroj: | Journal of Periodontology. 67:1267-1273 |
| ISSN: | 1943-3670 0022-3492 |
| DOI: | 10.1902/jop.1996.67.12.1267 |
| Popis: | We investigated the expression of interleukin-1 receptors (IL-1R) on the surfaces of cultured gingival fibroblasts derived from healthy and inflamed gingiva and the effects of IL-1α and IL-1β and prostaglandin E2 (PGE2) on IL-1R expression. Fibroblasts were obtained from expiant culture of both healthy and inflamed gingiva. IL-1R on cell surfaces was detected immunohistochemically using an anti-human IL-1R monoclonal antibody. IL-1R expression was assessed quantitatively using an enzyme linked immunosorbent assay (ELISA). Positive staining for IL-1R was more evident on cells from inflamed gingiva compared with cells from healthy gingiva. The ELISA showed a significantly increased number of IL-1R on cells from inflamed gingiva compared with cells from healthy gingiva (P < 0.05). Treatment with IL-1 but not PGE2 increased expression of IL-1R on fibroblasts. These findings suggest that gingival fibroblast responses to IL-1 may represent a mechanism for amplification of gingival inflammation. J Periodontol 19... |
| Databáze: | OpenAIRE |
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