Selection of low frequency tumor cells from cell culture by growth in nude mice
Autor: | Carole I. Bradt, Sidney M. Hopfer, F.W. Sunderman, Warren W. Nichols, Matthews O. Bradley, Rosina Hill, Andrew R. Kraynak |
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Rok vydání: | 1991 |
Předmět: |
Genetic Markers
Cancer Research medicine.medical_specialty Ratón Mice Nude Cell Count Tumor cells Biology Cytogenetics Mice Tissue culture Nude mouse In vivo Tumor Cells Cultured Genetics medicine Animals Molecular Biology Karyotype Neoplasms Experimental biology.organism_classification Kidney Neoplasms Rats Inbred F344 Rats Mice Inbred C57BL Cell culture Karyotyping Immunology Cancer research Cell Division Neoplasm Transplantation |
Zdroj: | Cancer Genetics and Cytogenetics. 53:57-66 |
ISSN: | 0165-4608 |
Popis: | Tissue cultures of tumor cells are frequently utilized to characterize chromosomal changes when direct cytogenetic preparations on tumors fail. The present study demonstrates that chromosomal markers found in direct tumor preparations can become undetectable in cell culture at variable rates presumably because of overgrowth of normal cell components in the culture. Injection of cultured tumor cells into nude mice followed by direct chromosomal preparations on the resulting nude mouse tumors can be used to select cells with the original tumor karyotype. This is true even when the tumor cell frequency in the culture is so low that they are not found in routine chromosomal preparations of the cultured cells. This technique can thus complement tissue culture findings and provide additional useful information about the original karyotype in cases where direct chromosomal preparations from tumors have failed. |
Databáze: | OpenAIRE |
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