Transgenic cattle produced by reverse-transcribed gene transfer in oocytes
| Autor: | E. J. Homan, L. U. Ballou, Jane C. Burns, Anthony W.S. Chan, Robert D. Bremel |
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| Rok vydání: | 1998 |
| Předmět: |
Cell division
Zygote Somatic cell Genetic Vectors Perivitelline space Biology Transfection Polymerase Chain Reaction Avian sarcoma virus Vesicular stomatitis Indiana virus Animals Genetically Modified Meiosis Pregnancy medicine Animals Mitosis Metaphase Repetitive Sequences Nucleic Acid Hepatitis B Surface Antigens Multidisciplinary Kanamycin Kinase Gene Transfer Techniques Biological Sciences Embryo Transfer Oocyte Molecular biology medicine.anatomical_structure Avian Sarcoma Viruses Oocytes Cattle Female Moloney murine leukemia virus |
| Zdroj: | Proceedings of the National Academy of Sciences. 95:14028-14033 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.95.24.14028 |
| Popis: | A critical requirement for integration of retroviruses, other than HIV and possibly related lentiviruses, is the breakdown of the nuclear envelope during mitosis. Nuclear envelope breakdown occurs during mitotic M-phase, the envelope reforming immediately after cell division, thereby permitting the translocation of the retroviral preintegration complex into the nucleus and enabling integration to proceed. In the oocyte, during metaphase II (MII) of the second meiosis, the nuclear envelope is also absent and the oocyte remains in MII arrest for a much longer period of time compared with M-phase in a somatic cell. Pseudotyped replication-defective retroviral vector was injected into the perivitelline space of bovine oocytes during MII. We show that reverse-transcribed gene transfer can take place in an oocyte in MII arrest of meiosis, leading to production of offspring, the majority of which are transgenic. We discuss the implications of this mechanism both as a means of production of transgenic livestock and as a model for naturally occurring recursive transgenesis. |
| Databáze: | OpenAIRE |
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